Rack1 binds HIV-1 Nef and can act as a Nef-protein kinase C adaptor

Virology
A GallinaG Milanesi

Abstract

Nef proteins of primate immunodeficiency viruses exert pleiotropic effects, such as enhanced endocytosis of CD4 and MHC-I cell surface molecules, perturbation of signal transduction cascades, and virion infectivity enhancement. Nef function intersects that of a number of cell kinases, including C kinases (PKCs) and Src-family kinases. Here the interaction of HIV-1 Nef with Rack1 (receptor for activated C kinase 1) is reported. Nef binds the Rack1 C-terminal moiety in a yeast two-hybrid system and in cell-free pull-down assays and copurifies with in vitro translated Rack1. Nef and Rack1 partially colocalize on the trans-Golgi network and plasma membranes. The presence of Rack1 doubles Nef phosphorylation by PKCs in vitro. Our data agree with the idea that Rack1 acts as a Nef intracellular docking site, bringing Nef and PKCs together. Other signal transduction or endocytosis proteins, in particular Src-like kinases, might meet Nef by intermediation of the Rack1 adaptor.

References

Nov 1, 1991·AIDS Research and Human Retroviruses·M A Gama SosaR M Ruprecht
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F GuillemotC Auffray
Aug 1, 1995·Journal of Virology·C Aiken, D Trono
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·S SalghettiJ Skowronski
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·D Ron, D Mochly-Rosen
Aug 1, 1994·Trends in Microbiology·M D MillerW C Greene
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·M H DisatnikD Mochly-Rosen
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·D RonD Mochly-Rosen
Jul 12, 1996·The Journal of Biological Chemistry·B L SmithP Berg
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·A SanfridsonC Doyle
May 9, 1997·The Journal of Biological Chemistry·K CoatesM P Harris
May 23, 1997·The Journal of Biological Chemistry·L X LiuS Benichou
Sep 23, 1997·Biochemical and Biophysical Research Communications·H WatanabeT Saito
Oct 6, 1997·The Journal of Cell Biology·M FotiJ L Carpentier
Jan 27, 1998·The Journal of Biological Chemistry·J Liliental, D D Chang
Apr 21, 1998·Advances in Pharmacology·D Mochly-Rosen, L M Kauvar
Aug 15, 1998·Trends in Cell Biology·J Oldridge, M Marsh
Apr 22, 1999·Protein Science : a Publication of the Protein Society·D R WestheadJ M Thornton
May 18, 1999·Archives of Biochemistry and Biophysics·J W Marsh

❮ Previous
Next ❯

Citations

Dec 1, 2009·Breast Cancer Research and Treatment·Xi-Xi CaoXiu-Ping Liu
Dec 31, 2011·Journal of Cancer Research and Clinical Oncology·Jing LiQianming Chen
May 15, 2012·FEBS Letters·Toby J Gibson
Feb 24, 2004·Journal of Cellular Physiology·Anna SeverinoAntonio De Luca
Feb 7, 2006·Progress in Neurobiology·Ella H SklanHermona Soreq
Dec 18, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Zhi WangQianming Chen
Jun 3, 2014·Oncogene·J-J Li, D Xie
Nov 19, 2002·Molecular Pharmacology·Angela McCahillStephen J Yarwood
Apr 25, 2002·The Journal of Biological Chemistry·Melanie TardifJean Gosselin
Feb 9, 2017·Applied Microbiology and Biotechnology·Yiling ZhangChengliang Gong
Dec 7, 2007·The Journal of Biological Chemistry·Ryuichi OhgakiHiroshi Kanazawa
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lina LuJianxiang Wu
Jan 1, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Marcello CeciNicla Romano
Mar 7, 2002·Microbes and Infection·Vivek K AroraJ Victor Garcia
Aug 6, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cheng QinJiyan Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.