RACK1 depletion in the ribosome induces selective translation for non-canonical autophagy.

Cell Death & Disease
Hag Dong KimJoon Kim

Abstract

RACK1, which was first demonstrated as a substrate of PKCβ II, functions as a scaffold protein and associates with the 40S small ribosomal subunit. According to previous reports, ribosomal RACK1 was also suggested to control translation depending on the status in translating ribosome. We here show that RACK1 knockdown induces autophagy independent of upstream canonical factors such as Beclin1, Atg7 and Atg5/12 conjugates. We further report that RACK1 knockdown induces the association of mRNAs of LC3 and Bcl-xL with polysomes, indicating increased translation of these proteins. Therefore, we propose that the RACK1 depletion-induced autophagy is distinct from canonical autophagy. Finally, we confirm that cells expressing mutant RACK1 (RACK1R36D/K38E) defective in ribosome binding showed the same result as RACK1-knockdown cells. Altogether, our data clearly show that the depletion of ribosomal RACK1 alters the capacity of the ribosome to translate specific mRNAs, resulting in selective translation of mRNAs of genes for non-canonical autophagy induction.

References

Sep 10, 1999·The Journal of Biological Chemistry·D RonA Gordon
Sep 11, 2002·Molecular Biology of the Cell·Eeva-Liisa EskelinenPaul Saftig
Nov 19, 2002·Molecular Pharmacology·Angela McCahillStephen J Yarwood
Sep 16, 2003·The Journal of Biological Chemistry·Boris ShorMaureen McLeod
Mar 5, 2004·Biochimica Et Biophysica Acta·Andrew M PetrosStephen W Fesik
Aug 31, 2004·Nature Structural & Molecular Biology·Jayati SenguptaJoachim Frank
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Eric H Baehrecke
Sep 24, 2005·Cell·Sophie PattingreBeth Levine
Feb 7, 2006·Progress in Neurobiology·Ella H SklanHermona Soreq
Sep 16, 2006·Molecular Aspects of Medicine·Eeva-Liisa Eskelinen
Jan 25, 2007·The EMBO Journal·Kassidy K HuynhSergio Grinstein
Apr 21, 2007·The EMBO Journal·M Chiara MaiuriGuido Kroemer
Jun 21, 2007·The Journal of Biological Chemistry·Serhiy PankivTerje Johansen
Jul 6, 2007·Autophagy·Noboru Mizushima, Tamotsu Yoshimori
Oct 7, 2008·Nature Cell Biology·Kyoko ArimotoMutsuhiro Takekawa
Dec 31, 2008·Molecular and Cellular Biology·Scott M CoyleJennifer A Doudna
Feb 17, 2009·Methods in Enzymology·Nina RabenPaul Plotz
Mar 13, 2010·The Journal of Pathology·Danielle GlickKay F Macleod
Apr 2, 2010·Trends in Cell Biology·Sarah F FunderburkZhenyu Yue
Jun 22, 2010·Nature·Christian BehrendsJ Wade Harper
Dec 30, 2010·Autophagy·Terje Johansen, Trond Lamark
Nov 8, 2011·Nature Structural & Molecular Biology·Seung Beom HongHyun Kyu Song
Dec 15, 2011·Nature Reviews. Molecular Cell Biology·Patrice CodognoTassula Proikas-Cezanne
Apr 7, 2012·International Journal of Cell Biology·Eduardo CebolleroClaudine Kraft
Apr 24, 2012·PloS One·Marcello CeciAntonino Cattaneo
May 24, 2012·Nature Reviews. Molecular Cell Biology·Shifeng Xue, Maria Barna
Jun 30, 2012·Cell Death and Differentiation·G Ashrafi, T L Schwarz
Dec 6, 2012·Cellular and Molecular Life Sciences : CMLS·Viviana VoltaStefano Biffo
Jan 9, 2013·Autophagy·Pui-Mun WongXuejun Jiang
Feb 19, 2013·Current Opinion in Microbiology·Konstantin ByrgazovIsabella Moll
Aug 6, 2013·Molecular Medicine Reports·Fangrong ShenYouguo Chen
Aug 15, 2013·The Plant Journal : for Cell and Molecular Biology·Corinna SpethSascha Laubinger
Dec 19, 2013·Genes & Cancer·Valentina GandinZe'ev A Ronai

❮ Previous
Next ❯

Citations

Feb 9, 2019·RNA·Max B Ferretti, Katrin Karbstein
May 2, 2018·EMBO Reports·Michal LubasLisa B Frankel
Jul 30, 2020·British Journal of Pharmacology·Erica BuosoMarco Racchi
Apr 2, 2020·Frontiers in Oncology·Pilar Sarah Acevo-RodríguezGreco Hernández
Jan 1, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Marcello CeciNicla Romano
Mar 24, 2020·Seminars in Cancer Biology·Mohd IshaqShrawan K Singh
Nov 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth I VinkIan Mohr

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
lipidation
co-IP
PCR
transfection

Software Mentioned

Operetta High - Content Imaging System
Harmony High - Content Imaging and Analysis

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms