RACK1 promotes maintenance of morphine-associated memory via activation of an ERK-CREB dependent pathway in hippocampus

Scientific Reports
Litao LiuLihong Wan

Abstract

Existence of long-term drug-associated memories may be a crucial factor in drug cravings and relapse. RACK1 plays a critical role in morphine-induced reward. In the present study, we used conditioned place preference (CPP) to assess the acquisition and maintenance of morphine conditioned place preference memory. The hippocampal protein level of RACK1 and synaptic quantitation were evaluated by Western blotting, immunohistochemistry and electron microscopy, respectively. Additionally, shRACK1 (shGnb2l1) was used to silence RACK1 in vivo to evaluate the role and the underlying mechanism of RACK1 in maintenance of morphine CPP memory. We found that morphine induced CPP was maintained for at least 7 days after the last morphine treatment, which indicated a positive correlation with hippocampal RACK1 level, and was accompanied simultaneously by increases in the synapse density and hippocampal expression of synaptophysin (SYP), phosphorylation of extracellular signal-regulated kinase1/2 (pERK1/2) and the phosphorylation of cyclic adenosine monophosphate response element-binding (pCREB). ShGnb2l1 icv injection significantly suppressed the expression of all above proteins, decreased the synapse density in the hippocampus and attenuated...Continue Reading

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Citations

Jan 13, 2017·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Chuang GuoZhan-You Wang
Nov 21, 2019·British Journal of Pharmacology·Li-Tao LiuLi-Hong Wan
Aug 31, 2017·Neural Regeneration Research·Leah Kershner, Kristy Welshhans
Jan 11, 2020·Frontiers in Cellular Neuroscience·Haihong YangHaitao Wu
Dec 11, 2019·Neuroscience and Biobehavioral Reviews·Corey B PuryearConnie Sanchez
Nov 3, 2020·Frontiers in Behavioral Neuroscience·Greer McKendrick, Nicholas M Graziane
Mar 16, 2021·Journal of Alzheimer's Disease : JAD·Ferdous TaslimaMakoto Michikawa
Apr 2, 2021·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Xueqin YanJun Dong

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Methods Mentioned

BETA
PCR
protein assay
electrophoresis
electron microscopy

Software Mentioned

Image Pro Plus
pro
SPSS
Image
Image J
GraphPad Prism
Nikon NIS - Elements D

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