Aug 15, 2013

RACK1 scaffold proteins influence miRNA abundance in Arabidopsis

The Plant Journal : for Cell and Molecular Biology
Corinna SpethSascha Laubinger

Abstract

MicroRNAs (miRNAs) regulate plant development by post-transcriptional regulation of target genes. In Arabidopsis thaliana, DCL1 processes precursors (pri-miRNAs) to miRNA duplexes, which associate with AGO1. Additional proteins act in concert with DCL1 (e.g. HYL1 and SERRATE) or AGO1 to facilitate efficient and precise pri-miRNA processing and miRNA loading, respectively. In this study, we show that the accumulation of plant microRNAs depends on RECEPTOR FOR ACTIVATED C KINASE 1 (RACK1), a scaffold protein that is found in all higher eukaryotes. miRNA levels are reduced in rack1 mutants, and our data suggest that RACK1 affects the microRNA pathway via several distinct mechanisms involving direct interactions with known microRNA factors: RACK1 ensures the accumulation and processing of some pri-miRNAs, directly interacts with SERRATE and is part of an AGO1 complex. As a result, mutations in RACK1 lead to over-accumulation of miRNA target mRNAs, which are important for ABA responses and phyllotaxy, for example. In conclusion, our study identified complex functioning of RACK1 proteins in the Arabidopsis miRNA pathway; these proteins are important for miRNA production and therefore plant development.

  • References62
  • Citations34

References

  • References62
  • Citations34

Citations

Mentioned in this Paper

Biochemical Pathway
PIWI Subfamily of Argonaute Proteins
Post-Transcriptional Regulation
Arabidopsis
Primary RNA Transcript
Hormone Receptors, Cell Surface
RNA, Small Temporal
Paracrine Protein Factors
AGO1 gene
Gene Expression Regulation, Plant

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