Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death

The EMBO Journal
E SonodaS Takeda

Abstract

Yeast rad51 mutants are viable, but extremely sensitive to gamma-rays due to defective repair of double-strand breaks. In contrast, disruption of the murine RAD51 homologue is lethal, indicating an essential role of Rad51 in vertebrate cells. We generated clones of the chicken B lymphocyte line DT40 carrying a human RAD51 transgene under the control of a repressible promoter and subsequently disrupted the endogenous RAD51 loci. Upon inhibition of the RAD51 transgene, Rad51- cells accumulated in the G2/M phase of the cell cycle before dying. Chromosome analysis revealed that most metaphase-arrested Rad51- cells carried isochromatid-type breaks. In conclusion, Rad51 fulfils an essential role in the repair of spontaneously occurring chromosome breaks in proliferating cells of higher eukaryotes.

References

Jul 1, 1978·Experimental Cell Research·J B SchvartzmanJ F López-Sáez
Aug 1, 1979·Molecular & General Genetics : MGG·D P Morrison, P J Hastings
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D A GordeninM A Resnick
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S TakedaJ M Buerstedde
Jan 1, 1992·Annual Review of Immunology·J J CohenK S Sellins
Apr 1, 1991·Current Genetics·Z ZgagaF Fabre
Sep 1, 1974·Mutation Research·J C Game, R K Mortimer
Jan 1, 1984·Molecular & General Genetics : MGG·R FingerhutF Otto
Apr 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S J GroteC A Shaw
Apr 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S J GroteC A Shaw
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R K MortimerD Schild
Aug 1, 1995·Immunology Today·O Vainio, B A Imhof
Oct 1, 1995·The Journal of Experimental Medicine·M TakataT Kurosaki
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·D E MyersF M Uckun
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T HaafD C Ward
Sep 25, 1993·Nucleic Acids Research·D F MurisA Pastink
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T MoritaA Matsushiro
Apr 11, 1993·Nucleic Acids Research·O BezzubovaJ M Buerstedde
Oct 1, 1995·Trends in Biochemical Sciences·A Shinohara, T Ogawa
Apr 24, 1996·Molecular & General Genetics : MGG·A YamamotoT Morita
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·L C HuangG M Wahl
Jun 14, 1996·Cell·A Fraser, G Evan
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Tsuzuki MoritaT
Jan 24, 1997·Cell·R ScullyD M Livingston
Feb 7, 1997·Cell·S Nagata

❮ Previous
Next ❯

Citations

Jan 1, 2006·The Arabidopsis Book·Hong Ma
Aug 30, 2000·International Journal of Cancer. Journal International Du Cancer·K YoshikawaR Takahashi
Nov 28, 2000·International Journal of Cancer. Journal International Du Cancer·H MaackeH W Stürzbecher
Aug 31, 2002·Genes, Chromosomes & Cancer·Elena KolomietzJeremy A Squire
Nov 6, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Thomas H O'Hare, Mary E Delany
Jun 14, 2005·Plant Molecular Biology·Keishi OsakabeSeiichi Toki
Aug 31, 2010·Irish Journal of Medical Science·E M Y Saleh, R A E El-Awady
Mar 16, 2005·Biochemical and Biophysical Research Communications·Ashish ShuklaBasuthkar J Rao
Dec 4, 2003·Mutation Research·Thomas Helleday
May 29, 2004·Mutation Research·Andrej Dudás, Miroslav Chovanec
Dec 10, 2002·Biochemical and Biophysical Research Communications·Yingzhu LiKen-ichi Yamamoto
Apr 25, 2003·Experimental and Molecular Pathology·Alexander J R Bishop, Robert H Schiestl
Feb 28, 2001·Journal of Immunological Methods·P Winding, M W Berchtold
Apr 23, 2003·Journal of Molecular Biology·Cecilia LundinThomas Helleday
May 30, 2001·Mutation Research·L H Thompson, D Schild
Aug 17, 2001·Mutation Research·A PastinkP H Lohman
Aug 14, 2003·Cell·Arkady CelesteAndré Nussenzweig
Sep 6, 2003·Trends in Genetics : TIG·Peter Hohenstein, Rachel H Giles
May 14, 1999·Trends in Genetics : TIG·J Thacker
Oct 3, 2002·Cellular Signalling·Fayza DaboussiBernard S Lopez
Dec 6, 2000·Mutation Research· van den Bosch MA Pastink
Jul 28, 1999·Mutation Research·F Ishikawa, T Naito
Oct 20, 1999·Mutation Research·A Shinohara, T Ogawa
May 10, 2000·Current Opinion in Cell Biology·J H Petrini
May 10, 2001·Current Opinion in Cell Biology·A R Venkitaraman
Jun 21, 2002·Trends in Cell Biology·Damien F HudsonWilliam C Earnshaw
Feb 13, 2001·Trends in Biochemical Sciences·J Y Masson, S C West
Aug 15, 2000·The International Journal of Biochemistry & Cell Biology·C Morrison, S Takeda
Oct 13, 1999·Mutation Research·A Pastink, P H Lohman
Feb 16, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Veronika FoltánkováEva Bártová
Mar 3, 2012·Journal of Medicinal Chemistry·Fei HuangAlexander V Mazin
Dec 19, 2006·Chemical Research in Toxicology·Michael D Wyatt, Douglas L Pittman
Dec 2, 2000·Nature·R Scully, D M Livingston
Oct 7, 2006·Nature·Zuzana StorchováDavid Pellman

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.