Raddeanin A, a natural triterpenoid saponin compound, exerts anticancer effect on human osteosarcoma via the ROS/JNK and NF-κB signal pathway

Toxicology and Applied Pharmacology
Bo MaQi Zhang

Abstract

Osteosarcoma (OS) is the most frequent and high mortality primary bone tumor in the adolescent. And it is well-known for poor prognosis due to high incidence of metastasis. Raddeanin A (RA), an active component of Anemone raddeana Regel, showed potential anti-cancer activities. However, the anti-tumor effect and molecular mechanism(s) of RA on osteosarcoma are still unclear. The present research is the first in vitro and in vivo investigate systematically anticancer of RA on human osteosarcoma. Our study demonstrated that RA induced mitochondria-dependent apoptosis in osteosarcoma cell lines and markedly suppressed the metastasis of osteosarcoma cells in vitro. And, RA treatment markedly inhibits tumor growth in vivo. Further mechanism study demonstrated that RA caused a significant enhance reactive oxygen species (ROS) level to stimulate phosphorylation of JNK. Moreover, RA led to decrease of p-IκBα level in the cytosol and reduction of p65 level in the nucleus, which was associated with the inhibition of NF-κB transcriptional activity. When NF-κB signaling was inhibited by siRNA targeting p65, a significant increase in cell apoptosis activity was observed. In addition, non-toxic RA concentrations (0.25, 0.5 and 1 μM) inhibite...Continue Reading

Citations

Feb 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irum NazKwang Seok Ahn
Mar 25, 2019·Journal of Cellular and Molecular Medicine·Hongyan XiaYan Dong
Jul 3, 2019·Natural Product Research·Chongning LvJincai Lu
Nov 9, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joo Tae HwangByoung Seob Ko
Aug 17, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Bo MaQi Zhang

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