Radial spoke proteins of Chlamydomonas flagella

Journal of Cell Science
Pinfen YangGeorge B Witman

Abstract

The radial spoke is a ubiquitous component of '9+2' cilia and flagella, and plays an essential role in the control of dynein arm activity by relaying signals from the central pair of microtubules to the arms. The Chlamydomonas reinhardtii radial spoke contains at least 23 proteins, only 8 of which have been characterized at the molecular level. Here, we use mass spectrometry to identify 10 additional radial spoke proteins. Many of the newly identified proteins in the spoke stalk are predicted to contain domains associated with signal transduction, including Ca2+-, AKAP- and nucleotide-binding domains. This suggests that the spoke stalk is both a scaffold for signaling molecules and itself a transducer of signals. Moreover, in addition to the recently described HSP40 family member, a second spoke stalk protein is predicted to be a molecular chaperone, implying that there is a sophisticated mechanism for the assembly of this large complex. Among the 18 spoke proteins identified to date, at least 12 have apparent homologs in humans, indicating that the radial spoke has been conserved throughout evolution. The human genes encoding these proteins are candidates for causing primary ciliary dyskinesia, a severe inherited disease invol...Continue Reading

References

Jan 11, 1979·The New England Journal of Medicine·J M SturgessJ A Turner
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G PipernoD J Luck
Sep 1, 1992·Molecular and Cellular Biology·A M CurryJ L Rosenbaum
May 1, 1988·The Journal of Cell Biology·N M Bonini, D L Nelson
Jul 1, 1986·The Journal of Cell Biology·B D WilliamsJ L Rosenbaum
Nov 1, 1967·Experimental Cell Research·M Gordon, R J Barrnett
Jul 1, 1981·Acta paediatrica Scandinavica·M AntonelliS Crifo
Jan 1, 1982·Progress in Clinical and Biological Research·R Kamiya
Jan 1, 1981·The Journal of Cell Biology·B HuangD J Luck
Dec 1, 1994·The Journal of Cell Biology·D R HowardW S Sale
Jun 1, 1994·The Journal of Cell Biology·M BernsteinJ L Rosenbaum
Jan 1, 1993·Cell Motility and the Cytoskeleton·A M Curry, J L Rosenbaum
Jan 1, 1996·Methods in Enzymology·A Lupas
Aug 15, 1997·The Journal of Biological Chemistry·S E BenashskiS M King
Jan 8, 1999·Molecular Biology of the Cell·C K OmotoG B Witman
Apr 30, 1999·Physiological Reviews·A L Fink
Jun 4, 1999·Trends in Cell Biology·M Colledge, J D Scott
Feb 26, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·E AsamizuS Tabata
Apr 4, 2000·Journal of Medical Genetics·M MeeksE Chung
Apr 11, 2000·European Journal of Human Genetics : EJHG·J L BlouinS E Antonarakis
Aug 19, 2000·Molecular Cell·H TakeshimaK Kangawa
Mar 10, 2001·Biochemical and Biophysical Research Communications·M ErikssonN Carey
Apr 20, 2001·The Journal of Cell Biology·A R GaillardW S Sale
Jun 13, 2001·The Journal of Cell Biology·P YangW S Sale
Jul 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lucia BartoloniStylianos E Antonarakis
Oct 25, 2002·Nature·Kevin M Ryan, Karen H Vousden
Oct 25, 2002·Nature·Hongwu ZhengZhi-Xiong Jim Xiao
Jan 9, 2003·Science's STKE : Signal Transduction Knowledge Environment·James H Hurley

❮ Previous
Next ❯

Citations

Mar 6, 2012·Nature Genetics·Hannah M MitchisonDavid R Mitchell
Aug 18, 2009·The Journal of Biological Chemistry·Arif JivanMelanie H Cobb
Aug 22, 2009·The Journal of Biological Chemistry·Pinfen YangStephanie Davis
May 27, 2011·Molecular Biology of the Cell·Erin E DymekElizabeth F Smith
Jun 29, 2012·Molecular Biology of the Cell·Thomas HeuserDaniela Nicastro
May 19, 2011·Molecular Human Reproduction·Kazuo Inaba
Jul 17, 2009·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Margaret W LeighMaimoona A Zariwala
May 12, 2009·Eukaryotic Cell·Jens BoesgerMaria Mittag
Jul 7, 2009·Annual Review of Microbiology·Katherine S RalstonKent L Hill
Aug 9, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anatoliy I MasyukNicholas F Larusso
Jan 15, 2013·International Journal of Plant Genomics·Jens BoesgerMaria Mittag
Jan 16, 2013·PloS One·Jessica M EsparzaSusan K Dutcher
May 22, 2008·Genetics·Kara L ChamberlainLaura R Keller
Oct 13, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hideo MohriShoji A Baba
Feb 27, 2014·American Journal of Respiratory and Critical Care Medicine·Michael R KnowlesMaimoona A Zariwala
Nov 7, 2013·Pediatric Research·Amjad HoraniThomas W Ferkol
Mar 5, 2014·The Journal of Cell Biology·Takashi Ishikawa
Mar 5, 2014·The Journal of Cell Biology·Toshiyuki OdaMasahide Kikkawa
Oct 31, 2007·The Journal of Cell Biology·Erin E Dymek, Elizabeth F Smith
Aug 26, 2009·The Journal of Cell Biology·Kaiyao HuangJoel L Rosenbaum
Nov 9, 2011·The Journal of Cell Biology·Gaia PiginoTakashi Ishikawa
Dec 17, 2009·The Journal of Cell Biology·Thomas HeuserDaniela Nicastro
Apr 26, 2011·Archives of Biochemistry and Biophysics·Maureen WirschellWinfield S Sale
Aug 7, 2010·Trends in Cell Biology·Rita TewariJuliet C Coates
Oct 20, 2015·Paediatric Respiratory Reviews·Amjad HoraniSteven L Brody
Nov 19, 2009·Reproduction in Domestic Animals = Zuchthygiene·J GuanL Yuan
Dec 25, 2007·Cell Motility and the Cytoskeleton·Maureen WirschellWinfield S Sale
Apr 24, 2008·Cell Motility and the Cytoskeleton·Hiroyuki Iwamoto
Mar 17, 2007·Annals of the New York Academy of Sciences·Kazuo Inaba

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.