Radially patterned cell behaviours during tube budding from an epithelium.

ELife
Yara E Sanchez-CorralesKatja Röper

Abstract

The budding of tubular organs from flat epithelial sheets is a vital morphogenetic process. Cell behaviours that drive such processes are only starting to be unraveled. Using live-imaging and novel morphometric methods, we show that in addition to apical constriction, radially oriented directional intercalation of cells plays a major contribution to early stages of invagination of the salivary gland tube in the Drosophila embryo. Extending analyses in 3D, we find that near the pit of invagination, isotropic apical constriction leads to strong cell-wedging. Further from the pit cells interleave circumferentially, suggesting apically driven behaviours. Supporting this, junctional myosin is enriched in, and neighbour exchanges are biased towards the circumferential orientation. In a mutant failing pit specification, neither are biased due to an inactive pit. Thus, tube budding involves radially patterned pools of apical myosin, medial as well as junctional, and radially patterned 3D-cell behaviours, with a close mechanical interplay between invagination and intercalation.

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Citations

Aug 25, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Matteo Rauzi
Jun 23, 2019·The Journal of Cell Biology·Clint S KoAdam C Martin
Jun 28, 2019·BMC Molecular and Cell Biology·Dorothy M Johnson, Deborah J Andrew
Sep 12, 2018·Nature·Guy Blanchard
May 21, 2020·Frontiers in Bioengineering and Biotechnology·Filippos IoannouJosé J Muñoz
Oct 30, 2020·Frontiers in Cell and Developmental Biology·Matthew P BostockVilaiwan M Fernandes
Jan 8, 2021·Development·Pedro Gómez-GálvezLuis M Escudero
Mar 13, 2021·Soft Matter·Kirsten D EndresenFrancesca Serra
Apr 22, 2021·Physical Biology·Clinton H Durney, James J Feng
Jun 8, 2021·Seminars in Cell & Developmental Biology·Giulia Paci, Yanlan Mao
Jun 27, 2021·Seminars in Cell & Developmental Biology·Alphy John, Matteo Rauzi
Jul 17, 2021·Cells & Development·Jessica C YuRodrigo Fernandez-Gonzalez
Jun 2, 2021·Seminars in Cell & Developmental Biology·Yu-Chiun Wang

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Software Mentioned

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