Radiation damage to nucleoprotein complexes in macromolecular crystallography

Journal of Synchrotron Radiation
Charles BuryJohn E McGeehan

Abstract

Significant progress has been made in macromolecular crystallography over recent years in both the understanding and mitigation of X-ray induced radiation damage when collecting diffraction data from crystalline proteins. In contrast, despite the large field that is productively engaged in the study of radiation chemistry of nucleic acids, particularly of DNA, there are currently very few X-ray crystallographic studies on radiation damage mechanisms in nucleic acids. Quantitative comparison of damage to protein and DNA crystals separately is challenging, but many of the issues are circumvented by studying pre-formed biological nucleoprotein complexes where direct comparison of each component can be made under the same controlled conditions. Here a model protein-DNA complex C.Esp1396I is employed to investigate specific damage mechanisms for protein and DNA in a biologically relevant complex over a large dose range (2.07-44.63 MGy). In order to allow a quantitative analysis of radiation damage sites from a complex series of macromolecular diffraction data, a computational method has been developed that is generally applicable to the field. Typical specific damage was observed for both the protein on particular amino acids and fo...Continue Reading

References

Jan 1, 1968·Advances in Protein Chemistry·G N Ramachandran, V Sasisekharan
Mar 5, 1999·Mutation Research·J CadetS Sauvaigo
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M WeikJ L Sussman
Mar 14, 2000·Acta Crystallographica. Section D, Biological Crystallography·W P Burmeister
Mar 25, 2000·Science·B D Michael, P O'Neill
Apr 4, 2000·Structure·R B Ravelli, S M McSweeney
Jun 14, 2001·International Journal of Radiation Biology·M BegusováM Spotheim-Maurizot
Apr 20, 2002·Biophysical Journal·Michel CharlierMélanie Spotheim-Maurizot
Apr 10, 2003·Journal of the American Chemical Society·Michael A HuelsLeon Sanche
May 20, 2004·Journal of the American Chemical Society·Joanna BerdysJack Simons
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 31, 2004·Radiation Research·Nathalie GillardMelanie Spotheim-Maurizot
Apr 21, 2005·Journal of Synchrotron Radiation·Colin Nave, Mark A Hill
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Elspeth F Garman, Robin Leslie Owen
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robin Leslie OwenElspeth F Garman
Aug 30, 2006·Current Opinion in Structural Biology·Raimond B G Ravelli, Elspeth F Garman
Oct 18, 2006·Accounts of Chemical Research·Jack Simons
Jan 11, 2007·Journal of Synchrotron Radiation·Emanuela FioravantiMartin Weik
Jan 11, 2007·Journal of Synchrotron Radiation·John E McGeehanRaimond B G Ravelli
Jan 19, 2007·Radiation Protection Dosimetry·Viktorie StísováMarie Davídková
Feb 1, 2007·The Biochemical Journal·Nathalie GillardMelanie Spotheim-Maurizot
May 16, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Sylwia Ptasińska, Léon Sanche
Feb 26, 2009·Journal of Synchrotron Radiation·Robin L OwenElspeth F Garman
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Aug 20, 2009·Acta Crystallographica. Section D, Biological Crystallography·N BallJ E McGeehan
Dec 24, 2009·Journal of Synchrotron Radiation·David FlotEdward Mitchell
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·Elspeth F Garman
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Feb 19, 2011·Mutation Research·M Spotheim-Maurizot, M Davídková
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ruslan SanishviliRobert F Fischetti
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·S McNicholasM E M Noble
Dec 21, 2012·Journal of Synchrotron Radiation·Elizabeth G AllanElspeth F Garman
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Oliver B ZeldinElspeth F Garman
Dec 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·P Therese LangTom Alber

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Citations

Feb 28, 2015·Journal of Synchrotron Radiation·Elspeth F Garman, Martin Weik
Apr 16, 2015·Journal of the American Chemical Society·Wei TangRichard B Silverman
May 4, 2016·Acta Crystallographica. Section D, Structural Biology·Zbigniew Dauter
May 4, 2016·Acta Crystallographica. Section D, Structural Biology·Charles S BuryElspeth F Garman
Dec 24, 2016·Journal of Synchrotron Radiation·Charles S BuryElspeth F Garman
Dec 24, 2016·Journal of Synchrotron Radiation·Elspeth F Garman, Martin Weik
Jul 6, 2019·Journal of Synchrotron Radiation·Elspeth F Garman, Martin Weik
Jul 6, 2019·Journal of Synchrotron Radiation·Valéria BugrisElspeth F Garman
Dec 4, 2019·Chemistry : a European Journal·Marwa AbdelmoulehJean-Christophe Poully
Dec 16, 2020·The Journal of Chemical Physics·Dipayan ChakrabortySylwia Ptasińska
May 1, 2018·Structure·Johan HattneTamir Gonen
Apr 22, 2021·Annual Review of Physical Chemistry·Karwan Ali OmarAurélien de la Lande

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Methods Mentioned

BETA
X-ray

Software Mentioned

PHASER
RADDOSE
FFT
CCP4mg
Coot
PHENIX
AIMLESS
CCP4
CAD
refine

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