Radiation effects on human leptomeningeal cell response to cerebrospinal fluid and PDGF-BB

International Journal of Radiation Biology
M D JohnsonJacqueline P Williams

Abstract

The pathogenesis of meningiomas remains largely unknown, but there is evidence that exposure radiation increases the risk of meningiomas. We therefore evaluated whether radiation stimulates human leptomeningeal cell proliferation and activates growth regulatory pathways. Cultures from six human primary leptomeningeal cells were established and irradiated with 0.5, 1 or 2 Gy. Cells were subsequently maintained for approximately 3-6 months and then treated with the mitogen, platlet-derived growth factor-BB (PDGF-BB), or human cerebrospinal fluid (CSF) obtained from patients with no neurological disease. Cell proliferation was evaluated by CyQUANT, and changes in the regulatory activation levels of signal transducer and activator of transcription (STAT3), mitogen-activated protein kinase kinase 1/2 (MEK1/2), mitogen activated protein kinase (MAPK 44/42), Akt, and mammalian target of rapamycin (mTOR) were assessed by Western blot. At both 3 and 6 months post-irradiation, CSF stimulated cell proliferation in all six leptomeningeal cultures. At 3 months, radiation was associated with an increase in STAT3, MEK 1/2, p44/42 MAPK and variable Akt activation, and at 6 months, STAT3, p44/42 MAPK, variable Akt and mTOR activation was observ...Continue Reading

References

Sep 30, 1991·International Journal of Cancer. Journal International Du Cancer·E F AdamsR Fahlbusch
Jan 1, 1995·British Journal of Neurosurgery·B S MusaB H Cummins
Feb 1, 1997·The British Journal of Radiology·R Doll, R Wakeford
Apr 30, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G B Van SettenG Schultz
Feb 12, 2000·American Journal of Epidemiology·S SadetzkiL Freedman
Jul 20, 2000·Journal of Neuropathology and Experimental Neurology·Y ShoshanJ K Cowell
Jul 29, 2000·Cancer Genetics and Cytogenetics·M J BelloJ A Rey
Jun 12, 2001·Journal of Neuropathology and Experimental Neurology·D X CaiA Perry
Nov 22, 2001·Experimental Neurology·B DattatreyamurtyV Chandrasekaran
Nov 27, 2002·Journal of Neurosurgery·Siegal SadetzkiDvora Nass
Mar 21, 2003·Cancer Genetics and Cytogenetics·Mamoru MurakamiKatsuyoshi Mineura
Aug 30, 2003·Oncogene·Paul DentSteven Grant
Jun 18, 2004·Journal of Neurosurgery·Ossama Al-MeftyMichael J Harrison
Oct 21, 2004·Neuro-oncology·Wolfgang K PfistererAdrienne C Scheck
Jun 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christian MawrinKnut Dietzmann
Jun 14, 2005·Cancer Cell·Goberdhan P Dimri
Sep 15, 2005·Cancer Genetics and Cytogenetics·S PiaskowskiPawel Piotr Liberski
May 12, 2006·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·E RotaP Ferrero
May 24, 2006·Molecular Pharmacology·Liwei ChenJie Wu
May 2, 2007·Journal of Immunotherapy·Marcin Kortylewski, Hua Yu
Oct 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Doris Germain, David A Frank
Dec 20, 2007·International Journal of Cancer. Journal International Du Cancer·Yanlei GuanKenshi Hayashi
Apr 17, 2008·Cancer Research·Anindya Bagchi, Alea A Mills
Jul 8, 2008·Acta Neuropathologica·Kevin O LilleheiB K Kleinschmidt-DeMasters
Jan 31, 2009·Neuro-oncology·Joanna BanerjeeArja Harila-Saari
Mar 19, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Seymour GruffermanHarold M Maurer
Apr 28, 2009·International Journal of Radiation Oncology, Biology, Physics·Arnold C PaulinoBin S Teh
Jul 14, 2009·Epidemiology·Anders AhlbomUNKNOWN ICNIRP (International Commission for Non-Ionizing Radiation Protection) Standing Committee on Epidemiology
Jul 21, 2009·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Raymond E MeynK Kian Ang
Nov 26, 2009·Acta Neuropathologica·Anna C NavisWiljan J A J Hendriks
Jul 17, 2010·Journal of the National Cancer Institute·Debra L FriedmanJoseph P Neglia
Sep 8, 2010·Journal of Neuro-oncology·Joseph WiemelsElizabeth B Claus

❮ Previous
Next ❯

Citations

May 23, 2014·International Journal of Radiation Biology·Pataje G S PrasannaC Norman Coleman

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.