PMID: 12779353Jun 5, 2003Paper

Radiation enhances the anti-tumor effects of vaccinia-p53 gene therapy in glioma

Technology in Cancer Research & Treatment
Tatyana M TimiryasovaIstvan Fodor

Abstract

The overall goal of this study was to analyze the effect and mechanism of radiation in combination with vaccinia viruses (VV) carrying the p53 gene against glioma. Comparison of two alternative treatments of cultured C6 (p53(+)) and 9L (p53(-)) rat glioma cells showed significantly reduced survival for both cell lines, especially 9L, when radiation was applied prior to virus versus radiation alone. High p53 protein expression mediated by VV-TK-p53 was measured in infected cells. Single modality treatment of C6 cells with psoralen and UV (PUV)-inactivated VV-TK-p53 (PUV-VV-TK-53) or radiation significantly decreased survival compared with PUV-inactivated L-15 (PUV-L-15) control virus. However, no difference was observed between radiation and combination treatments of C6 cells. In contrast, radiation followed by PUV-VV-TK-53 resulted in dramatic reduction of 9L cell viability, compared to single modality treatment. Flow cytometry analysis of Annexin-V-stained 9L cells showed that radiation and PUV-VV-TK-53 caused a significant decrease in live cells (17.2%) as compared to other treatments and control (61.6-98.3%). Apoptosis was observed in 37.2% of cells, while the range was 0.7-7.8% in other treatment groups; maximal p53 level w...Continue Reading

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Citations

Jan 12, 2005·The Journal of Urology·Istvan FodorMichael Lilly
Nov 3, 2009·Journal of Cancer Research and Clinical Oncology·Zhi-xiang YangXiao-hui Liu
Jun 27, 2006·Pathology Oncology Research : POR·Katalin Lumniczky, Géza Sáfrány
Oct 8, 2003·Technology in Cancer Research & Treatment·Kenneth Lundstrom, Teni Boulikas
Feb 23, 2011·Expert Opinion on Biological Therapy·Kilian GuseAkseli Hemminki
Dec 18, 2004·Critical Reviews in Clinical Laboratory Sciences·Severine SeemannPierre Hainaut
Mar 2, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sunil J AdvaniAladar A Szalay

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