Radiation enteropathy and leucocyte-endothelial cell reactions in a refined small bowel model

BMC Surgery
Louis Banka JohnsonBengt Jeppsson

Abstract

Leucocyte recruitment and inflammation are key features of high dose radiation-induced tissue injury. The inflammatory response in the gut may be more pronounced following radiotherapy due to its high bacterial load in comparison to the response in other organs. We designed a model to enable us to study the effects of radiation on leucocyte-endothelium interactions and on intestinal microflora in the murine ileum. This model enables us to study specifically the local effects of radiation therapy. A midline laparotomy was performed in male C57/Bl6 mice and a five-centimetre segment of ileum is irradiated using the chamber. Leucocyte responses (rolling and adhesion) were then analysed in ileal venules 2 - 48 hours after high dose irradiation, made possible by an inverted approach using intravital fluorescence microscopy. Furthermore, intestinal microflora, myeloperoxidase (MPO) and cell histology were analysed. The highest and most reproducible increase in leucocyte rolling was exhibited 2 hours after high dose irradiation whereas leucocyte adhesion was greatest after 16 hours. Radiation reduced the intestinal microflora count compared to sham animals with a significant decrease in the aerobic count after 2 hours of radiation. Fu...Continue Reading

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Citations

Jan 5, 2008·BMC Surgery·Andrea PolistenaBengt Jeppsson
Apr 14, 2009·Médecine sciences : M/S·Agnès FrançoisMarc Benderitter
Jan 27, 2009·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·L B JohnsonB Jeppsson
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Feb 18, 2011·The Journal of Surgical Research·Andrea PolistenaBengt Jeppsson
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Aug 17, 2021·Frontiers in Cellular and Infection Microbiology·Yongping JianZhi-Xiang Xu

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Methods Mentioned

BETA
fluorescence microscopy
light microscopy

Software Mentioned

CapImage

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