Radiation-induced chromosome aberrations in Saccharomyces cerevisiae: influence of DNA repair pathways.

Genetics
A A FriedlF Eckardt-Schupp

Abstract

Radiation-induced chromosome aberrations, particularly exchange-type aberrations, are thought to result from misrepair of DNA double-strand breaks. The relationship between individual pathways of break repair and aberration formation is not clear. By electrophoretic karyotyping of single-cell clones derived from irradiated cells, we have analyzed the induction of stable aberrations in haploid yeast cells mutated for the RAD52 gene, the RAD54 gene, the HDF1(= YKU70) gene, or combinations thereof. We found low and comparable frequencies of aberrational events in wildtype and hdf1 mutants, and assume that in these strains most of the survivors descended from cells that were in G2 phase during irradiation and therefore able to repair breaks by homologous recombination between sister chromatids. In the rad52 and the rad54 strains, enhanced formation of aberrations, mostly exchange-type aberrations, was detected, demonstrating the misrepair activity of a rejoining mechanism other than homologous recombination. No aberration was found in the rad52 hdf1 double mutant, and the frequency in the rad54 hdf1 mutant was very low. Hence, misrepair resulting in exchange-type aberrations depends largely on the presence of Hdf1, a component of t...Continue Reading

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Citations

Aug 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Juan Lucas ArguesoMichael A Resnick
Sep 30, 2008·Electromagnetic Biology and Medicine·M J Ruiz-GómezM Martínez-Morillo
Nov 14, 2000·Molecular and Cellular Biology·M KiechleR H Schiestl
Nov 22, 2005·Radiation Research·Rakesh Kumar Singh, Malini Krishna
Apr 24, 1999·Biochimie·F Eckardt-Schupp, C Klaus
Apr 3, 2021·International Journal of Environmental Health Research·Silvia Mercado-SáenzMiguel J Ruiz-Gómez
Jun 16, 2010·International Journal of Radiation Biology·Miguel J Ruiz-GómezManuel Martínez-Morillo
Oct 7, 2010·Electromagnetic Biology and Medicine·M J Ruiz-GómezM Martínez-Morillo

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