PMID: 15244377Jul 13, 2004Paper

Radiation-induced fragmentation of cardiolipin in a model membrane

International Journal of Radiation Biology
O I ShadyroJuergen Arnhold

Abstract

To obtain evidence for the possibility of free-radical fragmentation of cardiolipin under the action of ionizing radiation as measured by its aqueous dispersion from liposomes. Liposomes of tetramyristoylcardiolipin (TMCL) were exposed to gamma-rays from 60Co or 137Cs sources at doses between 1 and 24kGy. Fragmentation products were identified using thin-layer chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Using MALDI-TOF MS and thin-layer chromatography, it was shown that gamma-irradiation of liposomes consisting of TMCL was accompanied by free-radical fragmentation of the lipid to form dimiristoylphosphatidic acid and dimiristoylphosphatidyl hydroxyacetone. The yields of dimiristoylphosphatidic acid were greater than those of dimiristoylphosphatidyl hydroxyacetone, and formation of the named compounds was inhibited by dissolved oxygen. It is shown for the first time that on gamma-irradiation, cardiolipin can undergo free-radical fragmentation in its polar component.

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Citations

Jun 8, 2011·Chemistry and Physics of Lipids·Irina L YurkovaDominik Huster
Nov 6, 2008·Chemistry and Physics of Lipids·Irina YurkovaJuergen Arnhold
Aug 2, 2008·Chemistry and Physics of Lipids·M Rosário M DominguesPedro Domingues
Jun 15, 2013·Rapid Communications in Mass Spectrometry : RCM·Eliana AlvesAdelaide Almeida
Mar 9, 2005·Chemistry and Physics of Lipids·Irina YurkovaOleg Shadyro
Aug 18, 2005·Chemistry and Physics of Lipids·Irina YurkovaOleg Shadyro
Oct 4, 2011·Chemistry and Physics of Lipids·Beate FuchsJürgen Schiller
Sep 15, 2010·Free Radical Research·Corinne M SpickettNeven Zarkovic
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Elisabete MacielM Rosário M Domingues
Dec 31, 2010·Rapid Communications in Mass Spectrometry : RCM·Elisabete MacielM Rosário M Domingues
Oct 8, 2005·Journal of Radiation Research·Irina P EdimechevaTsutomu Kagiya
Dec 9, 2010·Lipids·Alexandra G LisovskayaIrina P Edimecheva
Aug 16, 2014·Journal of Proteome Research·Evagelia C LaiakisGiuseppe Astarita
Oct 7, 2008·Archives of Biochemistry and Biophysics·Irina L YurkovaDominik Huster

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