Radiation-induced interleukin-6 expression through MAPK/p38/NF-kappaB signaling pathway and the resultant antiapoptotic effect on endothelial cells through Mcl-1 expression with sIL6-Ralpha

International Journal of Radiation Oncology, Biology, Physics
Chia-Hung ChouJason Chia-Hsien Cheng

Abstract

To investigate the mechanism of interleukin-6 (IL-6) activity induced by ionizing radiation. Human umbilical vascular endothelial cells (HUVECs) were irradiated with different doses to induce IL-6. The IL-6 promoter assay and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to examine transcriptional regulation. Specific chemical inhibitors, decoy double-stranded oligodeoxynucleotides, and Western blotting were conducted to investigate the signal transduction pathway. Recombinant soluble human IL-6 receptor alpha-chain (sIL6-Ralpha) and specific small interfering RNA were used to evaluate the biologic function of radiation-induced IL-6. Four grays of radiation induced the highest level of IL-6 protein. The promoter assay and RT-PCR data revealed that the induction of IL-6 was mediated through transcriptional regulation. The p38 inhibitor SB203580, by blocking nuclear factor-kappaB (NF-kappaB) activation, prevented both the transcriptional and translational regulation of radiation-induced IL-6 expression. The addition of sIL6-Ralpha rescued HUVECs from radiation-induced death in an IL-6 concentratio-dependent manner. The antiapoptotic effect of combined sIL6-Ralpha and radiation-induced IL-6 was inhibited by mc...Continue Reading

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Citations

Sep 22, 2012·The Journal of Biological Chemistry·Mohammad HneinoFabien Milliat
Dec 19, 2014·Radiation Oncology·Elina Korpela, Stanley K Liu
Feb 24, 2015·Journal of Inflammation·Federica Maria Di MaggioValentina Bravatà
Oct 31, 2012·Radiation Research·Dörthe SchaueWilliam H McBride
Jul 17, 2016·Journal of Radiation Research·Soile Tapio
Jul 15, 2017·Scientific Reports·Cyprien JailletOlivier Guipaud
Feb 17, 2021·Clinical Research in Cardiology : Official Journal of the German Cardiac Society·Márta SárközyTamás Csont

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