Radiation-induced motility alterations in medulloblastoma cells

Journal of Radiation Research
Stefan RiekenStephanie E Combs

Abstract

Photon irradiation has been repeatedly suspected of increasing tumor cell motility and promoting locoregional recurrence of disease. This study was set up to analyse possible mechanisms underlying the potentially radiation-altered motility in medulloblastoma cells. Medulloblastoma cell lines D425 and Med8A were analyzed in migration and adhesion experiments with and without photon and carbon ion irradiation. Expression of integrins was determined by quantitative FACS analysis. Matrix metalloproteinase concentrations within cell culture supernatants were investigated by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using Student's t-test. Both photon and carbon ion irradiation significantly reduced chemotactic medulloblastoma cell transmigration through 8-μm pore size membranes, while simultaneously increasing adherence to fibronectin- and collagen I- and IV-coated surfaces. Correspondingly, both photon and carbon ion irradiation downregulate soluble MMP9 concentrations, while upregulating cell surface expression of proadhesive extracellular matrix protein-binding integrin α5. The observed phenotype of radiation-altered motility is more pronounced following carbon ion than photon irradiation. Both...Continue Reading

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Citations

Nov 22, 2017·Clinical & Experimental Metastasis·Benjamin J BlythOlga A Martin
Dec 13, 2016·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Arabinda DasRamin Eskandari
Mar 1, 2018·Brain : a Journal of Neurology·Veronica FerrucciMassimo Zollo

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Methods Mentioned

BETA
FCS
enzyme-linked immunosorbent assay
ELISA
fluorescence-activated cell sorting
X-ray
FACS

Software Mentioned

Magellan for F50
CellQuestPro

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