Radiation-induced muscle fibrosis rat model: establishment and valuation

Radiation Oncology
Yue ZhouXiao Zhou

Abstract

Lack of animal model of radiation induced muscle fibrosis, this study aimed to establish such a model by using 90 Gy single dose irradiation to mimic clinical relevance and also to explore the potential post-irradiation regenerative mechanism. SD rats were randomly divided into dose investigation groups and time gradient groups. Group1-6 were irradiated with a single dose of 65Gy, 70Gy, 75Gy, 80Gy, 85Gy and 90Gy respectively, and the degree of rectus femoris fibrosis in the irradiated area was detected at 4 weeks after irradiation. Group 7-9 were irradiated with a single dose of 90Gy, and the results were detected 1, 2, 4, and 8 weeks after irradiation. Then the general condition of rats was recorded. Masson staining was used to detect muscle fibrosis. The ultrastructure of muscles was observed by electron microscope, and the expression changes of satellite cell proliferation and differentiation related genes were detected by quantitative real-time-PCR. A single dose of 90Gy irradiation could cause muscle fibrosis in rats. As time goes on, the severity of muscle fibrosis and the expression of TGF- β1 increased. Significant swelling of mitochondria, myofilament disarrangement and dissolution, obvious endothelial cell swelling, i...Continue Reading

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Citations

Mar 2, 2019·Journal of Cellular and Molecular Medicine·Xiaowu ShengXiao Zhou
Sep 23, 2020·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Yoon KimJi Hye Hwang
Aug 28, 2020·Gastroenterology Report·Luca Reggiani BonettiFederica Domati
Aug 1, 2020·Clinical Orthopaedics and Related Research·Harvinder S Sandhu
Jul 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Anji XuXiaowu Sheng
Nov 30, 2021·Japanese Journal of Radiology·Yu MurakoshiRyota Iwasaki
Jan 13, 2022·ACS Biomaterials Science & Engineering·Żaneta GóreckaWojciech Święszkowski

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Methods Mentioned

BETA
biopsy
electron microscopy
electrophoresis
biopsies

Software Mentioned

- Blast
Pro Plus
Image
SPSS
Primer

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