Radiation Induces Apoptosis and Osteogenic Impairment through miR-22-Mediated Intracellular Oxidative Stress in Bone Marrow Mesenchymal Stem Cells

Stem Cells International
Zhonglong LiuYue He

Abstract

Bone marrow mesenchymal stem cells (BMSCs) were characterized by their multilineage potential and were involved in both bony and soft tissue repair. Exposure of cells to ionizing radiation (IR) triggers numerous biological reactions, including reactive oxygen species (ROS), cellular apoptosis, and impaired differentiation capacity, while the mechanisms of IR-induced BMSC apoptosis and osteogenic impairment are still unclear. Through a recent study, we found that 6 Gy IR significantly increased the apoptotic ratio and ROS generation, characterized by ROS staining and mean fluorescent intensity. Intervention with antioxidant (NAC) indicated that IR-induced cellular apoptosis was partly due to the accumulation of intracellular ROS. Furthermore, we found that the upregulation of miR-22 in rBMSCs following 6 Gy IR played an important role on the ROS generation and subsequent apoptosis. In addition, we firstly demonstrated that miR-22-mediated ROS accumulation and cell injury had an important regulated role on the osteogenic capacity of BMSCs both in vitro and in vivo. In conclusion, IR-induced overexpression of miR-22 regulated the cell viability and differentiation potential through targeting the intracellular ROS.

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Citations

Oct 28, 2019·Journal of Biomedical Materials Research. Part a·Aylin TahmasebiAbbas S Moghadam
May 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhonglong LiuYue He
May 8, 2020·International Journal of Molecular Medicine·Yang ZhouZhang Sun
Oct 16, 2019·Oxidative Medicine and Cellular Longevity·Gen-Xiang MaoJing Yan
Apr 28, 2019·International Journal of Environmental Research and Public Health·Amira BryllAndrzej Urbanik
Nov 4, 2021·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Partha MukhopadhyayRobert M Greene

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Methods Mentioned

BETA
X-ray
PCR
transfection
fluorescence microscopy
flow cytometry
Protein Assay
transfections
confocal microscopy
infrared spectroscopy
genetic modification

Software Mentioned

ImageJ
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis