PMID: 5920187Apr 1, 1966

Radiation-stimulated DNA synthesis in cultured mammalian cells

The Journal of Cell Biology
R E RASMUSSEN, R B Painter

Abstract

A type of DNA synthesis in mammalian cells that is stimulated by ultraviolet light has been studied by means of radioautography and density gradient centrifugation. The characteristics of this synthesis are: (a) it is not semiconservative; (b) it is enhanced by the presence of 5-bromodeoxyuridine in the DNA molecule; (c) the degree of stimulation is dose dependent; (d) there is less variability in the rate of incorporation of H(3)-thymidine during this synthesis than during normal DNA synthesis; (e) it occurs in cells that are not in the normal DNA synthesis phase (G(1) and G(2) cells). This kind of synthesis has been found in cultured cell lines from five different species; however, in some strains, the presence of bromouracil in the DNA is required before it can be demonstrated by radioautography.

References

Jul 15, 1965·Biochimica Et Biophysica Acta·D BillenG Jorgensen
Oct 1, 1960·Experimental Cell Research·R B PainterB G GIAUQUE
Aug 1, 1964·Journal of Molecular Biology·D PETTIJOHN, P C Hanawalt

Citations

Jan 1, 1969·Archiv für klinische und experimentelle Dermatologie·E G Jung, F Bay
Oct 1, 1972·Chemico-biological Interactions·W N BrandtN J Bernheim
Dec 1, 1971·Mutation Research·W G Connor, A Norman
Feb 1, 1972·Mutation Research·R B Painter, B R Young
Feb 1, 1973·Mutation Research·D Krishnan, R B Painter
Apr 1, 1974·Mutation Research·J M Clarkson, R B Painter
Mar 1, 1984·Mechanisms of Ageing and Development·E G NetteD W King
Nov 1, 1981·Mutation Research·E D ThompsonD Robinson
Oct 1, 1987·Mutation Research·B E ButterworthG M Williams
Oct 1, 1987·Mutation Research·B E ButterworthG M Williams
Dec 1, 1989·Mutation Research·L E HillG S Probst
Sep 1, 1986·Mutation Research·A Ootsuyama, H Tanooka
Nov 1, 1989·International Journal of Radiation Oncology, Biology, Physics·B S Dwarkanath, V K Jain
Jan 1, 1987·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S Rossberger, U Andrae
Mar 1, 1995·Mutation Research·P C Hanawalt
Sep 1, 1969·Biophysical Journal·A Han, W K Sinclair
Apr 17, 2002·Toxicology·Robert R Young
Sep 26, 2002·Mutation Research·Susanne Y Brendler-SchwaabBernd A Herbold
May 1, 1973·Quarterly Reviews of Biophysics·F Hutchinson
Oct 28, 1967·Nature·R B Painter, J E Cleaver
Jun 8, 1968·Nature·J J RobertsT P Brent
Feb 15, 1968·The New England Journal of Medicine·J B Little
Jun 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J E Cleaver
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S FakanR Hancock
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R W Hart, R B SETLOW
Jul 1, 1977·Journal of Toxicology and Environmental Health·B S StraussN P Higgins
Dec 1, 1968·Photochemistry and Photobiology·R F Kimball
Jul 1, 1970·Photochemistry and Photobiology·R B PainterB L Reisner
Nov 1, 1977·Photochemistry and Photobiology·J G Jose, K L Yielding
Aug 1, 1982·Photochemistry and Photobiology·R D WoodB Presson-Tincknell
Dec 1, 1973·Environmental Health Perspectives·W G Flamm
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J WidrowC D Laird
Jun 1, 1992·Scandinavian Journal of Gastroenterology·J FryklundH F Helander
Jun 1, 1973·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M L Meltz, R B Painter
Jan 1, 1971·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·L A Smets, J J Cornelis
Feb 1, 1972·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M Richold, C F Arlett
May 1, 1969·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R G Evans, B Djordjevic
Sep 1, 1988·International Journal of Radiation Biology·J S Rubin
Jan 1, 1970·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R E RASMUSSENR B Painter
Jan 1, 1968·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J B Little
Aug 1, 1977·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·V K JainS C Purohit
Jan 1, 1970·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M FoxB W Fox
Jan 1, 1969·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J E Cleaver
Jan 1, 1994·Environmental and Molecular Mutagenesis·P C Hanawalt
Jan 1, 1977·Progress in Histochemistry and Cytochemistry·W D Kuhlmann
Sep 1, 1976·International Journal of Radiation Oncology, Biology, Physics·J E ByfieldF Kulhanian
May 1, 1985·International Journal of Radiation Oncology, Biology, Physics·V K JainM Menon
Mar 1, 1977·Mutation Research·A R CollinsR T Johnson
Sep 2, 2003·Molecular Carcinogenesis·Brian S Thoma, Karen M Vasquez
Aug 27, 2010·Environmental and Molecular Mutagenesis·P C Hanawalt
Jul 1, 1973·Experimental Cell Research·T SekiguchiM Yamada
Oct 15, 1975·Experimental Cell Research·R A Pedersen, J E Cleaver
Mar 15, 1975·Experimental Cell Research·M WintzerithP Mandel
Dec 1, 1968·Journal of Theoretical Biology·G V DalrympleM L Baker
Nov 5, 1974·Journal of Molecular Biology·T SimmonsL D Hodge
Jul 1, 1989·Experimental Cell Research·E A Thomas, C A Thomas
Nov 1, 1971·Experimental Cell Research·S Kofman-Alfaro, A C Chandley
Jun 8, 1973·Biochemical and Biophysical Research Communications·B A Laishes, H F Stich
Nov 11, 1966·Biochemical and Biophysical Research Communications·G P StudzinskiJ L Schweitzer
May 1, 1978·Experimental Cell Research·E GiulottoF Nuzzo
Oct 1, 1972·Experimental Cell Research·Z DarzynkiewiczB G Arnason
Nov 1, 1972·Biochemical and Biophysical Research Communications·Y C LeeJ E Byfield
Aug 1, 1974·Toxicology and Applied Pharmacology·D R StoltzH C Grice
Nov 25, 1968·Biochemical and Biophysical Research Communications·D Roth
Feb 1, 1977·Experimental Eye Research·J G Jose, K L Yielding
Jan 1, 1989·Environmental and Molecular Mutagenesis·P C Hanawalt
Mar 1, 1987·Cytometry·K K Bose, D C Allison
Oct 1, 1985·Experimental Eye Research·J G Jose, D G Pitts
Jan 1, 1974·Journal of Theoretical Biology·C W Barnhill, K L Yielding
Jun 12, 2012·DNA Repair·Phil Hanawalt
Jan 1, 1995·The Journal of Dermatology·K Fukuyama
Dec 18, 2001·Environmental and Molecular Mutagenesis·P C Hanawalt
Dec 18, 2001·Environmental and Molecular Mutagenesis·R S TebbsL H Thompson
Oct 8, 2008·Annals of the New York Academy of Sciences·Peter B GahanMaurice Stroun
Nov 22, 1972·Nature: New Biology·A T HuangR B SETLOW
May 1, 2001·Current Protocols in Toxicology·J E Sutherland, M Costa
Jan 1, 1971·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·C D Lytle
Jan 1, 1969·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·H Barbason
Jan 1, 1968·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S StavrićF S Thatcher
Jul 1, 1969·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J E Cleaver, J E Trosko
Jan 1, 1967·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M Hill
Jan 1, 1970·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J F Scaife
Aug 15, 1975·International Journal of Cancer. Journal International Du Cancer·R H San, H F Stich
Sep 1, 1972·Experimental Cell Research·H Kato, T H Yosida

Related Concepts

Autoradiography
Centrifugation
DNA, Double-Stranded
HeLa Cells
Effects of Radiation
Thymidine
Black Light, Ultraviolet
Culture Techniques

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