A type of DNA synthesis in mammalian cells that is stimulated by ultraviolet light has been studied by means of radioautography and density gradient centrifugation. The characteristics of this synthesis are: (a) it is not semiconservative; (b) it is enhanced by the presence of 5-bromodeoxyuridine in the DNA molecule; (c) the degree of stimulation is dose dependent; (d) there is less variability in the rate of incorporation of H(3)-thymidine during this synthesis than during normal DNA synthesis; (e) it occurs in cells that are not in the normal DNA synthesis phase (G(1) and G(2) cells). This kind of synthesis has been found in cultured cell lines from five different species; however, in some strains, the presence of bromouracil in the DNA is required before it can be demonstrated by radioautography.
A novel technique for the detection of DNA single-strand breaks in human white blood cells and its combination with the unscheduled DNA synthesis assay
Enhancement of UV-induced unscheduled DNA synthesis by hydroxyurea. Demonstration by combined biochemical and autoradiographic techniques
Interphase sensitivity to the chromosome-breaking effect of 2'-deoxyadenosine: an autoradiographic study
Induction and rejoining of breaks in the deoxyribonucleic acid of human cells irradiated at various phases of the cell cycle
DNA-strand breaks and rejoining following exposure of synchronized Chinese hamster cells to ionizing radiations
Sensitivity of the newly synthesized and template DNA of lymphoma cells to damage by methyl methanesulphonate, and the nature of associated "repair" processes
Different inherited levels of DNA repair replication in xeroderma pigmentosum cell strains after exposure to ultraviolet irradiation
Effect of N-methyl-N'-nitro-N-nitrosoguanidine on the cell cycle and chromosomes of human embryonic lung cell
Kinetics of rejoining of single-strand breaks induced by ionizing radiation in DNA of human lymphocytes
The genetic effects of mitomycin C in Drosophila melanogaster. I. Induced mutations and X-Y chromosomal interchanges
Nature of chromosome aberrations induced in pre-Dna-synthesis period in human leukocytes by daunomycin
Unscheduled DNA synthesis in human leucocytes after exposure to UV light, -rays and chemical mutagens
Caffeine potentiation of the chromosome damage produced in bean root tips and in Chinese hamster cells by various chemical and physical agents
Studies on DNA repair in frog and human cells exposed to an acridine half-mustard (ICR 191) and to MNNG
An autoradiographic study of unscheduled DNA synthesis in the germ cells of male mice treated with X-rays and methyl methanesulfonate
Single-strand breaks in DNA during repair of UV-induced damage in normal human and xeroderma pigmentosum cells as determined by alkaline DNA unwinding and hydroxylapatite chromatography: effects of hydroxyurea, 5-fluorodeoxyuridine and 1-beta-D-arabinofuranosylcytosine on the kinetics of repair
Induction of DNA repair synthesis by ultraviolet radiation and methylmethanesulphonate in cultured mouse lymphocytes
Correlation between deoxyribonucleic acid excision-repair and life-span in a number of mammalian species
Repair replication: a density-gradient analysis of DNA from cells infected with mengovirus or Newcastle disease virus
Mitotic G 2 delay induced in synchronized human kidney cells by UV and x-irradiation and its relation to DNA strand breakage, repair and transcription
Repairable and irrepairable damage in 5-bromouracil-substituted DNA exposed to ultra-violet radiation
Effect of non-toxic concentrations of acriflavine on unscheduled DNA-synthesis and survival of synchronized HeLa cells exposed to ultra-violet light
Radiation-induced DNA degradation in human cells: lack of evidence following moderate doses of x-rays
Inhibition of unscheduled DNA synthesis and repair of potentially lethal X-ray damage by 2-deoxy-D-glucose in yeast
Fully functional global genome repair of (6-4) photoproducts and compromised transcription-coupled repair of cyclobutane pyrimidine dimers in condensed mitotic chromatin
Effects of 2-deoxy-D-glucose on glycolysis, proliferation kinetics and radiation response of human cancer cells
Accumulation of nuclear DNA damage or neuron loss: molecular basis for a new approach to understanding selective neuronal vulnerability in neurodegenerative diseases
Correlation of cell division and specific protein production during the course of lymphoid cell differentiation
Changing levels of uv light and carcinogen-induced unscheduled DNA synthesis in mouse oocytes during meiotic maturation
Repair synthesis and sedimentation analysis of DNA of human cells exposed to dimethylnitrosamine and activated dimethylnitrosamine
Carcinogenesis in tissue culture. X. Rejoining of single-strand breaks in DNA by mammalian cells induced by chemical carcinogens (4-nitroquinoline 1-oxide and its derivative) in vitro
Elevation of deoxyribonuclease activities in HeLa cells treated with selective inhibitors of DNA synthesis
Genotoxicity and cytotoxicity of selected pyrrolizidine alkaloids, a possible alkenal metabolite of the alkaloids, and related alkenals
Do mammalian cells repair radiation injury by the "cut-and-patch" mechanism? II. An extension of the original model
DNA repair synthesis in mammalian cells exposed to a series of oncogenic and non-oncogenic derivatives of 4-nitroquinoline 1-oxide
Host-cell reactivation in mammalian cells. I. Survival of ultra-violet-irradiated herpes virus in different cell-lines
Human monocytes and lymphocytes differ in the rate of DNA repair synthesis and viability after exposure to nitrogen mustard
Effects of gamma-irradiation on Escherichia coli wild type and its radiation-resistant mutants. II. Post-irradiation degradation of DNA
RNA synthesis and uridine pools in normal and BUdR-containing human kidney T-cells after U.V.-irradiation
Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.
Alzheimer's Disease: MS4A
Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.
Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.
Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.
Genetic Screens in iPSC-derived Brain Cells
Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.
This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.
Chronic Fatigue Syndrome
Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.
Pharmacology of Proteinopathies
This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.
Alignment-free Sequence Analysis Tools
Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.