Radiation target analyses of DNA template/primer complexes

Biophysical Journal
Nicolas Sluis-Cremer, Ellis Kempner

Abstract

Frozen solutions of low molecular weight DNA template/primer complexes, in the absence and presence of HIV-1 reverse transcriptase, were irradiated with high-energy electrons. Molecules that survived the radiation exposure were quantified and analyzed using radiation target theory. Transfer of radiation-deposited energy was observed by the damage caused. It was found that damage (as a polynucleotide chain break) was observed in one chain when the radiation interaction occurred in the other chain, suggesting a transfer of energy. In contrast, the target sizes of the DNA template/primers were not altered if bound to HIV-1 reverse transcriptase, signifying that the deposited radiation energy is not transferred between protein and nucleic acid.

References

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Jan 1, 1985·Methods in Enzymology·J T HarmonE S Kempner
Nov 1, 1995·Bulletin of Mathematical Biology·E S Kempner
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Jun 25, 2002·Journal of Molecular Biology·Curt A DaveyTimothy J Richmond
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Nicolas Sluis-CremerMichael A Parniak

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Citations

Nov 26, 2008·Analytical Biochemistry·T J AnchordoquyE S Kempner

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