Radio-immunoassay for formyl methionyl leucyl phenylalanine. II. Demonstration of an enterohepatic circulation of immunoreactive bacterial chemotactic peptides in man

Journal of Gastroenterology and Hepatology
E C RobertsV S Chadwick

Abstract

Bacterial chemotactic peptides (F-met-oligopeptides) are secreted by several species of commensal enteric bacteria and can be assayed by bioassay techniques in human colonic luminal fluid. We have previously demonstrated intestinal absorption and enterohepatic circulation of radiolabelled F-met peptides introduced into rat colon, and an eightfold increase in absorption and biliary excretion in rats with experimental colitis. This paper describes the application of a radio-immunoassay to measurements of formyl oligopeptides in human faecal dialysates, colonic and systemic venous blood and bile. All samples were fractionated by reverse-phase high performance liquid chromatography (HPLC) prior to assay. Immunoreactivity was found in faecal dialysates (5-700 nmol/L F-met-leu-phe equivalents) and bile samples (3-150 nmol/L) from normal subjects. After HPLC fractionation, up to five distinct peaks of immunoreactivity were identified. One of these co-chromatographed with authentic F-met-leu-phe; the others probably represented either closely related peptides or peptides of different chain lengths originating from the same F-met-leu-phe precursor protein. Colonic venous blood from two patients with ulcerative colitis contained immunore...Continue Reading

References

Jul 1, 1989·Gastroenterology·D M FerryV S Chadwick
Jan 1, 1988·Scandinavian Journal of Gastroenterology. Supplement·B StrandvikH Glaumann

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Citations

Feb 1, 1992·Digestive Diseases and Sciences·R P AndersonV S Chadwick
Mar 5, 2013·The Journal of Clinical Investigation·Keqiang ChenJi Ming Wang
Nov 15, 2014·Journal of Leukocyte Biology·Giovanna LeoniAlgirdas J Jesaitis
Apr 30, 2019·Journal of Thrombosis and Haemostasis : JTH·Maryam F SalamahSakthivel Vaiyapuri
Apr 5, 2012·Paediatric and Perinatal Epidemiology·Wei PerngEduardo Villamor
Jul 23, 2013·The Journal of Biological Chemistry·Walid S MaatyAlgirdas J Jesaitis
Jul 31, 2019·Reproduction in Domestic Animals = Zuchthygiene·Natalia G C VasileiouGeorge C Fthenakis
Nov 10, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R A VilchezS Kusne
Feb 27, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Regis A VilchezShimon Kusne
Mar 5, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Regis A VilchezShimon Kusne
Jun 29, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michael G Ison
Jan 6, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bo WeiJonathan Braun
Mar 29, 2003·Scandinavian Journal of Immunology·K LangF Entschladen

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