Radio-isotopic determination of subnanomolar amounts of peroxide

Free Radical Research Communications
S D Varma

Abstract

A new radio-isotopic method for determination of peroxide in subnanomolar concentrations has been described. The method is based on the reactivity of peroxide with alpha-Ketoglutaric acid containing [I-14C]-alpha-Ketoglutaric acid, and measurement of the resulting 14CO2 by radioactivity. The recovery of standard peroxide, alone or mixed with some biologically derived samples, following this technique was found to be 97 +/- 2.7%. The method may therefore be useful for determination of peroxides in active biological samples.

References

Jan 1, 1988·Free Radical Research Communications·S D Varma, S M Morris
Jun 30, 1934·The Journal of Experimental Medicine·M G Sevag, L Maiweg

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Citations

Jul 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B HalliwellO I Aruoma
Jun 25, 2002·Molecular Aspects of Medicine·Wilhelm StahlSiân B Astley
Jun 25, 2002·Molecular Aspects of Medicine·Helen R GriffithsSiân B Astley
Jan 1, 1990·Free Radical Research Communications·S D Varma, P S Devamanoharan
Jan 1, 1991·Free Radical Research Communications·S D Varma, P S Devamanoharan
Jan 1, 1990·Free Radical Research Communications·B Halliwell
Apr 8, 1999·Free Radical Research·N KugeT Sato
Sep 1, 1991·Current Eye Research·P S DevamanoharanS D Varma
May 17, 1995·Biochemical Pharmacology·B Halliwell
Oct 1, 1991·Experimental Eye Research·S RamachandranS D Varma
Sep 2, 1999·Biochemical and Biophysical Research Communications·L H LongB Halliwell
Nov 7, 2002·Biological & Pharmaceutical Bulletin·Kazuyuki HiramotoKiyomi Kikugawa

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