PMID: 8474581May 1, 1993Paper

Radio-label and mass determinations of inositol 1,3,4,5-tetrakisphosphate formation in rat cerebral cortical slices: differential effects of myo-inositol

Neurochemical Research
P KurianF T Crews

Abstract

To investigate the effects of increasing concentrations of myo-inositol (inositol) on receptor stimulated [3H]inositol polyphosphate formation in the absence of lithium, slices of rat cerebral cortex were incubated with various concentrations of [3H]inositol (1 to 30 microM). Carbachol stimulated formation of [3H]inositol trisphosphate (InsP3) and [3H]inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4) increased several fold when the inositol concentration was increased reaching a plateau at approximately 12 microM inositol. Time course studies revealed that in the presence of low concentrations of inositol (1 microM), [3H]InsP3 and [3H]Ins(1,3,4,5)P4 formation in response to carbachol stimulation increased slowly over a 10 to 20 min time period, whereas in the presence of 4 and 12 microM inositol, carbachol stimulated [3H]InsP3 and [3H]Ins(1,3,4,5)P4 formation was rapid and essentially complete within 3 to 5 min after carbachol addition. Although the carbachol dose response in 12 microM inositol had a much greater maximal efficacy, there was no change in potency. Similar to the effects of carbachol on [3H]Ins(1,3,4,5)P4 formation from prelabeled phosphoinositides, muscarinic receptor stimulation increased Ins(1,3,4,5)P4 mass f...Continue Reading

References

Jul 1, 1992·Kidney International·N J Guzman, F T Crews
Feb 1, 1991·Alcoholism, Clinical and Experimental Research·L J ChandlerF T Crews
Jan 1, 1990·Annual Review of Cell Biology·V S Bansal, P W Majerus
Jan 1, 1985·Analytical Biochemistry·C J van DongenW H Gispen
Aug 1, 1987·The Biochemical Journal·K A WreggettR F Irvine
Sep 1, 1984·Analytical Biochemistry·L C MacGregor, F M Matschinsky

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