PMID: 6103864Apr 1, 1980Paper

Radioactive labelling of toxin I from Anemonia sulcata and binding to crayfish nerve in vitro

Hoppe-Seyler's Zeitschrift für physiologische Chemie
S Stengelin, F Hucho

Abstract

1. Radioactive derivatives of neurotoxin I (ATX I) from Anemonia sulcata have been synthesized: Iodination of ATX I with 125I yielded a mixture of reaction products from which monoiodo and diiodo ATX I were isolated. 2. 125I-ATX I was shown to bind to the axonal membrane from Astacus leptodactylus main walking nerve. Specificity of binding was shown by saturability of the binding sites and by competitive binding of native and radioactive toxin. 3. Astacus nerve bound 44 fmol of 125I-ATX I/mg nerve (wet weight). The axonal membrane surface of the nerve was determined to be 7800 cm2/g nerve. This amounts to a binding site density of around 35/mu2 axonal surface. Binding was not inhibited by tetrodotoxin, the blocker of the selectivity filter of voltage-dependent sodium channels. 125I-ATX I therefore may bind to the sodium channel-inactivating gate. 4. The affinity of the nerve membrane receptors for 125I-ATX I appears to be voltage-dependent: KD = 5 nM was found with whole crayfish nerves in the presence of tetrodotoxin, KD = 40nM in the absence of tetrodotoxin and an even lower affinity was obtained with axonal membrane fragments isolated from the nerve. Drugs destabilizing the membrane potential, e.g. veratridine, ouabain and s...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W A Catterall, C S Morrow
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J M Ritchie, R B Rogart
Jan 1, 1977·Annual Review of Biophysics and Bioengineering·W Ulbricht
Dec 29, 1977·Molecular and Cellular Biochemistry·F Hucho, W Schiebler
Nov 1, 1972·The Journal of Cell Biology·A L Hubbard, Z A Cohn

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Citations

Jan 1, 1988·Pflügers Archiv : European journal of physiology·A WarashinaT Ogura
Jul 28, 1981·Biochimica Et Biophysica Acta·P AshG Wunderer
Jan 1, 1983·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·S FujitaM Satake
Mar 1, 1983·The Journal of General Physiology·A Warashina, S Fujita
May 15, 1981·Analytical Biochemistry·S StengelinF Hucho

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