PMID: 4292091Jun 1, 1967Paper

Radioautographic localization of the increased synthesis of phosphatidylinositol in response to pancreozymin or acetylcholine in guinea pig pancreas slices

The Journal of Cell Biology
L E HOKIN, D Huebner

Abstract

A technique is described for measuring the incorporation of myo-inositol-2-(3)H into the lipid of various regions of the guinea pig pancreatic acinar cell by radioautography. Stimulation of enzyme secretion with either pancreozymin or acetylcholine was associated with increased graining in both the basophilic cytoplasm and the nonbasophilic cytoplasm. Kinetic studies suggested that the incorporation of myo-inositol-2-(3)H was stimulated independently in the two regions. Most of the increment in graining due to stimulation with pancreozymin or acetylcholine plus eserine was abolished if the tissue was extracted with 2:1 chloroform-methanol before radioautography. On chromatography of lipid extracts of pancreas, the only lipid showing a detectable increment in radioactivity on stimulation with pancreozymin was phosphatidylinositol. Thus, essentially all of the increment in graining is likely to be due to increased incorporation of tritium into phosphatidylinositol. These studies, coupled with earlier studies employing differential centrifugation, indicate that on stimulation of enzyme secretion there is increased synthesis of phosphatidylinositol in the rough-surfaced endoplasmic reticulum and in the smooth-surfaced Golgi membran...Continue Reading

References

Jun 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·L E Hokin
Feb 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J D Jamieson, G E Palade
Feb 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L E HOKIN, M R HOKIN
Oct 1, 1959·The Journal of Biophysical and Biochemical Cytology·C M REDMAN, L E HOKIN

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Citations

Jan 1, 1986·The Journal of Membrane Biology·M C Sekar, L E Hokin
Mar 1, 1968·Currents in Modern Biology·J M Vasiliev, I M Gelfand
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P J MarshallL E Hokin
May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Feb 1, 1971·Journal of Cellular Physiology·D H Lockwood, H G Williams-Ashman
May 15, 1988·Biochemical Pharmacology·B A Chapman, N R Pattinson
Feb 1, 1972·Journal of Ultrastructure Research·S K KimS S Han
Nov 1, 1978·Prostaglandins and Medicine·J M GerrardJ G White
Oct 17, 1969·Annals of the New York Academy of Sciences·L E Hokin
Oct 1, 1977·Archives internationales de physiologie et de biochimie·G Dandrifosse
Mar 1, 1973·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·Hadar Emanuelsson
Jan 1, 1985·The American Journal of Physiology·D SchlondorffJ A Satriano
Mar 25, 1975·Biochimica Et Biophysica Acta·R H Michell

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