PMID: 6984816Jan 1, 1982Paper

Radioautographic study of "in vitro" incorporation of [3H]-hydroxyindoles and [3H]-melatonin into the pineal organ of the pigeon

Archives d'anatomie microscopique et de morphologie expérimentale
P VoisinJ P Collin

Abstract

In the pigeon pineal organ studied in vitro, high resolution radioautography was used to visualize the sites of uptake and retention of [3H]-indoles: --incubation of the pineal organ with [3H]-5-hydroxytryptophan ([3H]-5-HTP) during the day and with [3H]-5-hydroxytryptamine ([3H]-5-HT) during the night led to strong selective labelling of the receptor line cells (RLC) which correspond to modified photoreceptor cells (rudimentary photoreceptor cells). No significant radioautographic reactions were found in other cell types. When the 20 min radioactive signal was followed by 20-100 min post-incubation in a cold medium, labelling was still restricted to RLC. The in vitro study of indole metabolism sites might be facilitated by this preparation. --labelling after [3H]-melatonin incorporation was found mainly in RLC but also in interstitial cells. Reactions were less intense than those obtained with [3H]-hydroxyindoles although some erythrocytes were strongly labelled. No definite conclusion can be drawn from the radioautographs, in which artefacts were apparent.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.