Radiobiological characteristics of solid tumours depending on the p53 status of the tumour cells, with emphasis on the response of intratumour quiescent cells

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
S MasunagaM Takagaki

Abstract

Human head and neck squamous cell carcinoma cells transfected with mutant TP53 (SAS/mTP53) or with a neo vector as a control (SAS/neo) were inoculated subcutaneously (s.c.) into both hind legs of Balb/cA nude mice. Mice bearing tumours received 5-bromo-2'-deoxyuridine (BrdU) continuously to label all proliferating (P) cells in the tumours. The mice then received gamma-ray irradiation. Another group of mice received a series of test doses of gamma-rays while alive or after tumour clamping to obtain hypoxic fractions (HFs) in the tumours. Right after irradiation, the tumour cells were isolated and incubated with a cytokinesis blocker. The micronucleus (MN) frequency in the cells without BrdU labelling (=quiescent (Q) cells) was determined using immunofluorescence staining for BrdU. Meanwhile, 6 h after irradiation, tumour cell suspensions obtained in the same manner were used for determining the frequency of apoptosis in the Q cells. The MN frequency and apoptosis frequency in total (P+Q) tumour cells were determined from the tumours that were not pretreated with BrdU. In total cell populations, SAS/mTP53 cells were more radioresistant than SAS/neo cells in clonogenic survival. Q tumour cells exhibited a significantly lower apopt...Continue Reading

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Citations

Sep 9, 2009·International Journal of Radiation Oncology, Biology, Physics·Yang HanEn-Hua Wang
Jan 18, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katherine E ChapmanGareth J S Jenkins
Nov 14, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Dharmarajan SriramPerumal Yogeeswari
Aug 14, 2003·British Journal of Cancer·G DriessensC Bruyns
Apr 3, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Peter EbbesenAndrea Scozzafava

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