PMID: 9539574Apr 16, 1998Paper

Radiobiological hypoxia in the KHT sarcoma: predictions using the Eppendorf histograph

International Journal of Radiation Oncology, Biology, Physics
D W SiemannM R Horsman

Abstract

To investigate whether electrode measurements of tumor oxygenation obtained under a range of different treatment conditions designed to alter the degree of tumor hypoxia could be correlated with estimates of radiobiological hypoxia measured under the same conditions. Experiments were performed in restrained, nonanesthetized, female C3H/He mice, which had approximately 0.5 g KHT sarcomas growing intramuscularly in the hind limbs. The treatments used to modify tumor oxygenation status included breathing gas mixtures of varying oxygen content, altering tumor blood flow, and shifting the hemoglobin oxygen dissociation curve. Radiobiological hypoxic fraction was estimated using the paired survival curve assay, while electrode measurements of tumor oxygenation were obtained with an Eppendorf histograph. With the selected manipulations it was possible to vary the radiobiological hypoxic fraction in the tumors from approximately 1 to approximately 100% of the total viable cell population. Furthermore, these changes in radiation response were directly reflected in the changes in tumor oxygenation measurements made with the Eppendorf histograph. These findings suggest that in the KHT tumor model the Eppendorf electrode measurements could...Continue Reading

References

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Citations

Jun 7, 2005·International Journal of Radiation Oncology, Biology, Physics·Dietmar W Siemann, Amyn M Rojiani
Oct 18, 2000·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·C Aquino-ParsonsA I Minchinton
Feb 9, 2000·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·T H KnockeR Pötter
Aug 5, 2000·International Journal of Radiation Oncology, Biology, Physics·A P van den BergA G Visser
May 15, 2002·International Journal of Radiation Oncology, Biology, Physics·Dietmar W Siemann, Amyn M Rojiani
Dec 9, 1998·International Journal of Radiation Oncology, Biology, Physics·L LiD W Siemann
Jul 27, 1999·International Journal of Radiation Oncology, Biology, Physics·M C KavanaghR P Hill
Feb 8, 2000·International Journal of Radiation Oncology, Biology, Physics·A BeckerJ Dunst
Dec 3, 2002·International Journal of Radiation Oncology, Biology, Physics·Dietmar W Siemann, Amyn M Rojiani
Dec 14, 2004·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Howard W Salmon, Dietmar W Siemann
Apr 6, 2004·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Michael R Horsman, Jens Overgaard
Feb 4, 2006·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Lynsey A HuxhamAndrew I Minchinton
Nov 9, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Atsuko MatsumotoKen-Ichiro Matsumoto
Sep 29, 2006·Colloids and Surfaces. B, Biointerfaces·Yuanfang Liu, Shaopeng Wang

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