Radioenzymatic assay of platelet serotonin, dopamine and norepinephrine in subjects with normal and increased serotonin production

Clinica Chimica Acta; International Journal of Clinical Chemistry
J M Feldman, J A Davis

Abstract

Platelet serotonin, dopamine and norepinephrine concentrations were determined with sensitive and specific radioenzymatic methods in normal volunteers and in sick subjects with both normal and increased serotonin production. In the assays, a 3H-labeled methyl group is transferred from [3H]S-adenosyl methionine to the monoamine under investigation by a semi-purified enzyme. The platelet serotonin concentration (expressed as pmol serotonin/mg platelet protein) was significantly higher in the patients with increased serotonin production from carcinoid tumors (5400 +/-0 500) than in the normal volunteers (900 +/- 100) or the sick subjects with normal serotonin production (900 +/- 100). Although eight of the subjects with increased serotonin production had symptoms of the carcinoid syndrome such as flushing and diarrhea, two of the subjects with increased serotonin production did not have these symptoms. The radioenzymatic method of platelet serotonin measurement was as effective as a conventional fluorometric technique for serum serotonin in detecting patients with serotonin overproduction from carcinoid tumors, and had the advantages of being more sensitive and specific. The great sensitivity and specificity of the radioenzymatic ...Continue Reading

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Citations

Sep 1, 1987·Journal of Autism and Developmental Disorders·J M LaunayJ Fermanian
Dec 24, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·J M Feldman, C Klatt
Sep 16, 1982·The New England Journal of Medicine·S S Farag, D B Gelles
Mar 1, 1985·Metabolism: Clinical and Experimental·J M Feldman
Oct 1, 1988·The American Journal of Medicine
Mar 1, 1995·The American Journal of Cardiology·P A RobiolioJ M Feldman
Dec 22, 1986·The American Journal of Medicine·J M Feldman, T M O'Dorisio
May 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Andrew J VickersBarrie R Cassileth
Nov 1, 1985·International Journal of Dermatology·E Mooney
May 1, 1984·Metabolism: Clinical and Experimental·J M FeldmanR E Coleman
Apr 1, 1985·European Journal of Cancer & Clinical Oncology·F Engbaek
Sep 18, 1997·Diseases of the Colon and Rectum·M A Memon, H Nelson
Dec 1, 1989·Current Problems in Surgery·J M Feldman

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