PMID: 8968732Nov 1, 1996Paper

Radioimagers as an alternative to film autoradiography for in situ quantitative analysis of 125I-ligand receptor binding and pharmacological studies

The Histochemical Journal
M Crumeyrolle-AriasF Haour

Abstract

Three radioimagers, the mu-imager, the beta-imager and the phosphorimager, were tested as alternatives to quantitative autoradiography on film, for receptor imaging and pharmacological in situ quantitative analysis. Two iodinated ligands 125I-interleukin-1 alpha and 125I-gonadotropin releasing hormone agonist were used for receptor characterization in mouse brain and pituitary sections. Due to the high number of the agonist receptors in rat pituitary gland, this tissue was used to compare measurements obtained from digital autoradiograms with classical gamma detector determination. This permits the evaluation of radioimager efficiency and absolute quantification. Radioimagers represent an improvement in terms of time of image acquisition. All the radioimagers are more sensitive than film for the detection of low levels of radioactivity. The spatial resolution provided by the mu-imager compares favourably with that obtained on film autoradiograms while digital autoradiograms from the phosphorimager and beta-imager did not show precise definition under our experimental conditions. Superimposition of histological structures from the stained sections with radiolabelled areas in the autoradiograms remains, at this time, the unique a...Continue Reading

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Citations

Dec 24, 1998·Nuclear Medicine and Biology·Y CharonH Tricoire
Jun 18, 2002·Journal of Neurochemistry·G BanisadrS Mélik Parsadaniantz
Jun 18, 1998·Annals of the New York Academy of Sciences·F HaourT Ternynck
Sep 18, 2007·Biomolecular Engineering·C CholletM C Durrieu
Apr 28, 2005·Brain Research·Alexei MedvedevVivette Glover
Jan 21, 2006·Journal of Neuroscience Methods·Géraldine PoisnelDanièle Debruyne
Feb 1, 2003·Journal of Neurochemistry·Michèle Crumeyrolle-AriasVivette Glover
Dec 14, 2007·Developmental Neuroscience·Françoise Phan Dinh TuyFiona Francis

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