PMID: 6159552Nov 13, 1980Paper

Radioimmune, radiobinding and HPLC analysis of 2-5A and related oligonucleotides from intact cells

Nature
M KnightI M Kerr

Abstract

The enzyme (2-5A synthetase) which synthesizes ppp(A2'p)nA where n=2 to 4 (collectively referred to as 2-5A) is widely distributed in a variety of cells and tissues in amounts which increase response to interferon and vary with growth and hormone status. 2-5A activates a nuclease which inhibits protein synthesis. The non-phosphorylated 'core' of 2-5A ((A2'p)nA, n=2 to 4) can inhibit DNA synthesis and cell growth. Here we describe convenient and sensitive radioimmune (RI) and radiobinding (RB) assays for core and 2-5A. In combination with more satisfactory high performance liquid chromatography (HPCL) methods using reverse-phase C18 columns, these assays have been used to detect core and 2-5A in crude extracts from interferon-treated cells. The novel 2-5A synthetase products NAD2'p5' A2'p5'A and A5'p45'A2'p5'A2'p5'A (ref. 13), which can also be detected using the RB assay, were not found in significant amounts. The natural occurrence of core has not been described previously.

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A KimchiM Revel
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I M Kerr, R E Brown
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E SlatteryP Lengyel
Oct 12, 1978·Nature·T E England, O C Uhlenbeck
Feb 10, 1977·Nature·W G Light, E T Wei
May 1, 1976·Journal of Virology·I M KerrD Baltimore
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R SilberJ Hurwitz
Aug 13, 1974·Biochemistry·R M D'Alisa, B F Erlanger
Nov 5, 1980·Journal of Molecular Biology·M A ConklingJ W Drake
Jul 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B F ERLANGER, S M BEISER

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Citations

Jan 1, 1992·Biomedical Chromatography : BMC·H Suzuki, D T Buonamassa
Aug 30, 1984·Biochemical and Biophysical Research Communications·W K RobertsI M Kerr
Jan 1, 1984·Pharmacology & Therapeutics·G C Sen
Dec 1, 1994·Journal of Virological Methods·B A Hassel, P O Ts'o
Oct 18, 1983·Biochimica Et Biophysica Acta·T J BorelliJ M Wu
Jun 1, 2005·Experimental and Molecular Pathology·Marc FrémontPatrick Englebienne
Jun 12, 1998·Pharmacology & Therapeutics·M R Player, P F Torrence
May 16, 1998·Gene·A Benoit De CoignacT Salehzada
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H CaillaJ Marti
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Etienne-SmekensJ E Dumont
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H JacobsenR H Silverman
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L LaurenceH Cailla
Aug 5, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·F Le RoyC Bisbal
Jul 11, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M TnaniB Bayard
May 1, 1982·European Journal of Biochemistry·R H SilvermanI M Kerr
Sep 1, 1982·European Journal of Biochemistry·M Verhaegen-Lewalle, J Content
Apr 15, 1983·European Journal of Biochemistry·M C HaughI M Kerr
Sep 2, 1985·European Journal of Biochemistry·B BayardB Lebleu
Nov 16, 1987·European Journal of Biochemistry·M A TrujilloH V Rickenberg
Feb 15, 1989·European Journal of Biochemistry·C BisbalB Bayard
May 1, 1989·Biological Reviews of the Cambridge Philosophical Society·T Alderson
Jul 19, 2011·PLoS Neglected Tropical Diseases·Matty KnightWannaporn Ittiprasert
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Chandar S ThakurRobert H Silverman
Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Krishnamurthy MalathiRobert H Silverman
Sep 11, 2012·Cellular and Molecular Life Sciences : CMLS·Alys Peisley, Sun Hur
Jul 1, 1985·Experimental Cell Research·V Wells, L Mallucci

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