PMID: 6162915Mar 1, 1981Paper

Radioimmunoassay for central nervous system myelin-specific proteolipid protein

Journal of Neurochemistry
J L TrotterH C Agrawal

Abstract

A double-antibody radioimmunoassay (RIA) has been developed with antisera to purified rat brain myelin proteolipid protein (PLP). The addition of Triton X-100 allowed antibody-antigen interaction and immune precipitation in the presence of sodium dodecyl sulfate (SDS). The RIA will accurately measure 8-80 ng of PLP in buffer or human serum. The RIA is highly specific for myelin PLP and does not cross-react with material in tissues (heart, kidney, muscle, testicle, and intestine) other than the central nervous system. The antibodies to rat myelin PLP cross-react with PLP from bovine brain homogenate or myelin. Myelin PLP was found to account for 55 and 52% of total myelin protein from bovine and rat brain, respectively. Furthermore, there is a higher concentration of PLP in white than in gray matter corresponding to the degree of myelination. Unlike myelin basic protein, myelin PLP was undetectable in both bovine and rat peripheral nervous system.

References

Apr 10, 1978·Biochimica Et Biophysica Acta·J M Boggs, M A Moscarello
Mar 1, 1977·Journal of Neurochemistry·H C AgrawalF L Margolis
Jul 1, 1970·Journal of Neurochemistry·F Gonzalez-Sastre
Apr 23, 1974·Biochemical and Biophysical Research Communications·J L NussbaumP Jollès
Sep 1, 1972·Journal of Neurochemistry·H C AgrawalA L Prensky
Dec 7, 1971·Biochemistry·J GagnonM A Moscarello

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Citations

Jan 1, 1984·Neurochemical Research·J L TrotterW F Garvey
Jan 1, 1984·Neurochemical Research·J L TrotterW W Tourtellotte
Jun 19, 1992·Brain Research. Developmental Brain Research·A KondoH Nagara
Sep 18, 2008·Journal of Neuroscience Research·Mihaela AniteiRashmi Bansal
Nov 1, 1982·Journal of Neurochemistry·D JohnsonR O Brady
Apr 1, 1996·Multiple Sclerosis : Clinical and Laboratory Research·C M PelfreyH F McFarland
Jan 1, 1982·Journal of Neuroscience Research·W B MacklinM B Lees

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