Radioimmunoassay for measurement of bovine alpha-lactalbumin in serum, milk and tissue culture media

The Journal of Dairy Research
R M AkersH M Farrell

Abstract

A sensitive, specific radioimmunoassay for bovine alpha-lactalbumin (alpha-la) suitable for measurement of serum, tissue culture media and milk was developed. alpha-La was not detected in serum from prepubertal male or female cattle, but was detected as early as 60 d of gestation in nulliparous Holstein heifers, the level being greatly increased during the last one third of gestation. Serum from cross bred beef heifers contained less alpha-la and it was not detected until late gestation. Concentrations of alpha-la in serum samples from pregnant multiparous Holstein cows decreased at drying-off and subsequently increased just before parturition. Secretion of alpha-la by mammary tissue explants from steroid-primed prepubertal Holstein heifers was induced by the addition of bovine prolactin, ovine prolactin or human growth hormone to tissue culture media.

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Citations

Jan 1, 1987·The International Journal of Biochemistry·L M SordilloS P Oliver
Jan 1, 1989·The International Journal of Biochemistry·J J SmithR M Akers
Jan 1, 1988·Tissue & Cell·C R BaumruckerR M Akers
Oct 1, 1987·Domestic Animal Endocrinology·K PlautR M Akers
Jul 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C A GibsonC W Welsch
Sep 1, 1992·Research in Veterinary Science·C G ProsserS R Davis
Jun 16, 2010·Journal of Animal Physiology and Animal Nutrition·A PezeshkiC Burvenich
May 16, 2014·Journal of Animal Physiology and Animal Nutrition·A StarkC Baumrucker
Mar 1, 2013·Balsaeng'gwa saengsig·Yoon-Young Sung, Chul-Sang Lee
Feb 1, 1987·Journal of Dairy Science·T B McFaddenG W Kazmer
Nov 1, 1990·Journal of Dairy Science·K M KuzmanoffC W Beattie
Nov 1, 1988·Journal of Dairy Science·T B McFaddenW E Beal
Oct 1, 1994·Journal of Dairy Science·M A GuyT E Besser
Jan 1, 1997·Journal of Dairy Science·G M BarringtonT B McFadden
Dec 5, 2000·Journal of Dairy Science·S BallR M Akers
Jun 1, 1989·Journal of Dairy Science·S P Oliver, L M Sordillo
Mar 1, 1988·Journal of Dairy Science·T B McFaddenA V Capuco
Mar 21, 2006·Annals of Biomedical Engineering·Julie M D Paye, Kimberly Forsten-Williams
Oct 1, 1996·Current Opinion in Biotechnology·Y Echelard

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