PMID: 3746204Jun 1, 1986Paper

Radioimmunoassay for the measurement of insulin-like growth factor I in patients with pituitary disease in comparison with commercially available somatomedin-C radioimmunoassays

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
B PfeifleH Ditschuneit

Abstract

A highly sensitive and specific radioimmunoassay for the measurement of insulin-like growth factor I (IGF I) has been developed. The IGF I concentrations were measured in sera of normal subjects, patients with acromegaly, hypophysectomized patients and patients with hyperprolactinaemia. The results were compared with the results obtained after measurement of IGF I in the sera of the same patients with two commercially available radioimmunoassays for somatomedin-C. IGF I was separated from its carrier protein using Sep-Pak C18 cartridges. The total recovery of IGF I by this method was about 100%. The in-house assay shows a high specificity for IGF I and a high sensitivity. As little as 0.1 microgram/l of IGF I can be detected. Only a small amount of serum (25 microliter) is necessary for the IGF I determination and a great number of serum samples (more than 100 per day) can be processed. The mean immunoreactive IGF I concentration was 379 +/- 159 micrograms/l in normal adult subjects, 3340 +/- 1094 micrograms/l in acromegalic patients, and 52 +/- 11 micrograms/l in growth-hormone deficient patients. Patients with hyperprolactinaemia had an IGF I concentration of 433 +/- 112 micrograms/l.

References

Sep 1, 1977·The Journal of Clinical Investigation·R W FurlanettoA J D'Ercole
Nov 1, 1977·The Journal of Clinical Endocrinology and Metabolism·R L Hintz, F Liu
Jan 1, 1976·Hormone Research·K Reber, R Liske
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·D J KnauerG L Smith
Jul 1, 1981·The Journal of Clinical Endocrinology and Metabolism·R M WhiteR E Johnsonbaugh
Aug 1, 1982·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·B PfeifleH Ditschuneit

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