PMID: 489926Apr 1, 1979Paper

Radioimmunoassay measurement of prostaglandins E2 and F2alpha in human urine

Journal of Endocrinological Investigation
G CiabattoniC Patrono

Abstract

Techniques are described in detail for the radioimmunological measurement of prostaglandin (PG) E2 and F2alpha in human urine. 50 ml urine samples are extracted with an organic solvent system and then purified by silicic acid column chromatography. The overall recovery after extraction and purification, calculated with labeled as well as unlabeled compounds, is in the order of 70%. The column eluates are assayed at 1:12-1:60 dilution in the "standard diluent" of the assay: 8 pg PGE2 or PGF2 alpha/ml of whole urine represents the lowest measurable concentration. A urine blank and a solvent blank were evaluated separately, subjecting 50 ml of urine obtained from an indomethacin treated subject ("PG-free" urine) or 50 ml of distilled water, respectively, to the extraction-purification procedures. Both were found not to interfere with the antigen-antibody reaction. Urinary PG-like immunoreactivity (LI) was characterized in terms of immunochemical and thin-layer chromatographic (TLC) behavior. Both urinary PGE2-LI and PGF2 alpha-LI behaved as authentic PGE2 and PGF2 alpha, upon dilution and on TLC. In 33 healthy female subjects (aged 19-58 yr), urinary excretion rates averaged 178 +/- 80 (mean +/- SD) ng/day for PGE2 and 498 +/- 181...Continue Reading

References

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Citations

May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bianca RoccaCarlo Patrono
Feb 5, 2003·Current Medical Research and Opinion·Stefania TacconelliPaola Patrignani
Mar 1, 1990·Kidney International·C ZojaG Remuzzi
Sep 1, 1995·Clinical Pharmacology and Therapeutics·F CipolloneP Patrignani
Apr 1, 1997·The Journal of Laboratory and Clinical Medicine·S CastellaniG Masotti
Oct 29, 1987·Biochemical and Biophysical Research Communications·P PatrignaniP Musiani
Apr 6, 2007·Fertility and Sterility·Francesca MiniciRosanna Apa
Oct 23, 2001·International Journal of Cancer. Journal International Du Cancer·F O RanellettiL Lauriola
Aug 1, 1986·Hypertension·L M RuilopeJ C Romero
Jul 15, 1998·Clinical Pharmacology and Therapeutics·M R PanaraP Patrignani
Jul 1, 1990·Annals of the Rheumatic Diseases·M SasanoK Nishioka
Mar 1, 1989·Annals of the Rheumatic Diseases·N MiyasakaK Nishioka
Mar 1, 1995·Scandinavian Journal of Urology and Nephrology·M LazzeriD Turini
Feb 25, 2003·British Journal of Pharmacology·Maria G SciulliPaola Patrignani
Jun 1, 1982·Annals of the Rheumatic Diseases·I CarusoM C Gandini
Jul 22, 2010·International Journal of Immunopathology and Pharmacology·L MenghiniL Leporini
Jul 4, 2015·British Journal of Pharmacology·Luigia Di FrancescoPaola Patrignani
Feb 1, 1984·Journal of Endocrinological Investigation·M MattioliL A Scuro
Apr 18, 2012·International Journal of Immunopathology and Pharmacology·M PetriniG Spoto
Jun 15, 2020·Biochemical Pharmacology·Stefania TacconelliPaola Patrignani
Jun 6, 1983·Life Sciences·M DonlonG N Catravas
Nov 28, 1996·European Journal of Pharmacology·G SantiniP Patrignani
Jul 1, 1988·Biochimica Et Biophysica Acta·M LivioG Remuzzi

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