Radioimmunoassay of 17-hydroxyprogesterone in serum with or without thin-layer chromatographic purification.

Hormone Research
E J Wickings

Abstract

A radioimmunoassay for the measurement of 17-hydroxyprogesterone (17-OHP) is described. The antigen 11-desoxycortisol-21-hemisuccinate-bovine serum albumin has been used to produce two antisera of different antibody populations in the same animal. The thin-layer chromatographic system described can be used to separate all the cross-reacting steroids investigated from 17-OHP. A simplified method is also presented using preliminary solvent extraction only. The mean 17-OHP levels measured, using this method, were, for normal men, 0.86 +/- 0.19 ng/ml (SD), for normal females 0.30 +/- 0.19 ng/ml in the follicular phase and 1.72 +/- 0.18 ng/ml in the luteal phase of the menstrual cycle, and 0.45 +/- 0.17 ng/ml in normal postmenopausal women.

Citations

Apr 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·B A el-Gamal, J Landon
May 1, 1979·Journal of Steroid Biochemistry·J Fiet, M T Pham-Huu-Trung
Mar 1, 1987·Journal of Steroid Biochemistry·B A el-GamalL A Perry
Oct 1, 1988·Scandinavian Journal of Clinical and Laboratory Investigation·B A el-GamalJ Landon
Jul 19, 1978·Archiv für Gynäkologie·D MühlenstedtH P Schneider
Jan 1, 1988·Annals of Clinical Biochemistry·B A el-GamalJ Landon

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