Radioimmunoassay of a newt sex pheromone, sodefrin, and the influence of hormones on its level in the abdominal gland

General and Comparative Endocrinology
K YamamotoSakaé Kikuyama

Abstract

A specific and sensitive radioimmunoassay (RIA) was developed for the measurement of sodefrin, a female-attracting decapeptide pheromone of the red-bellied newt, Cynops pyrrhogaster. Sodefrin synthesized according to the amino acid sequence of native sodefrin, isolated from the abdominal glands of the cloaca of the male newt, was used as a reference standard. An antiserum to sodefrin was produced by immunizing a rabbit with synthetic sodefrin that was extended on its C-terminus with Cys coupled to hemocyanin. For the radioligand, sodefrin N-terminally extended with Tyr was used. An aqueous extract of the abdominal glands of C. pyrrhogaster produced a displacement curve parallel to the sodefrin standard, whereas that from the sword-tailed newt (Cynops ensicauda) showed no inhibition of binding in this RIA. The sensitivity of the RIA was 30.5 +/- 3.4 pg/100 microliters assay buffer. Intraassay and interassay coefficients of variation were 1.6 and 4.5%, respectively. The RIA was used to determine sodefrin levels in the abdominal gland of the male newt. Hypophysectomy and castration greatly reduced the sodefrin content. Administration of testosterone propionate (TP) to the hypophysectomized and castrated newt increased the pheromon...Continue Reading

Citations

Mar 6, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Aya KodaKenichiro Kitamura
Feb 8, 2011·General and Comparative Endocrinology·Masatoshi MitaYoshitaka Nagahama
May 14, 2005·Annals of the New York Academy of Sciences·Yoichi ItoSakaé Kikuyama
May 14, 2005·Annals of the New York Academy of Sciences·Sakae KikuyamaJ Michael Conlon
May 22, 2009·Annals of the New York Academy of Sciences·Sakae KikuyamaKazuyoshi Tsutsui
May 29, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hiroshi MatsukawaSakae Kikuyama
Jun 4, 2008·Zoological Science·F Toyoda, S Kikuyama
Jun 20, 2014·Hormones and Behavior·Sarah Woodley
Sep 18, 2004·Peptides·Fumiyo ToyodaSakae Kikuyama
Mar 8, 2005·General and Comparative Endocrinology·Fumiyo ToyodaSakaé Kikuyama
Nov 14, 2016·General and Comparative Endocrinology·Kazutoshi YamamotoMasatoshi Mita
Apr 28, 2005·Cell and Tissue Research·Itaru HasunumaSakae Kikuyama
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·S KikuyamaA M Polzonetti-Magni
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kostadin AndonovBorislav Naumov
Feb 17, 2001·General and Comparative Endocrinology·N TakahashiS Kikuyama
Jul 5, 2005·Hormones and Behavior·Frank L MooreDarcy B Kelley

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