PMID: 107972Jan 1, 1979

Radioimmunoassay of carbonic anhydrase I and II in rats. Application to the central nervous system during ontogeny

Biochimie
N LimozinG Laurent

Abstract

A specific radioimmunoassay method for rat erythrocyte carbonic anhydrases I and II was developed using a double antibody system. Its sensitivity was in the nanogram range for each of the two isozymes. The method has been applied to the assay of cerebral carbonic anhydrase. Only CAII has been found in brain extracts of perfused rats. Accordingly, the assay of CAI in cerebral tissue can be used to quantify erythrocyte contamination on condition that the ratio CAII/CAI in blood had been worked out. The developmental change in the soluble and the Triton X-100 solubilized brain CAII from birth to adult is reported.

Citations

Jul 1, 1982·Calcified Tissue International·T Kumpulainen, H K Väänänen
Jul 1, 1980·The Histochemical Journal·O K LangleyG Gombos

Related Concepts

Senility
Metazoa
Brain
Carbonate Dehydratase
Alloenzymes
Radioimmunoassay
Rats, Laboratory

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