PMID: 6979038Jan 1, 1982Paper

Radioimmunoassay of CRF-like material in rat hypothalamus

Peptides
R L Moldow, A J Fischman

Abstract

Corticotropin releasing factor (CRF) was recently isolated from ovine hypothalami by its ability to stimulate adrenocorticotropin (ACTH) and beta-endorphin release from dispersed rat pituitary cells. In order to study the physiology of this peptide, we have developed a sensitive and specific radioimmunoassay (RIA) for CRF. Synthetic CRF was conjugated to bovine thyroglobulin and emulsified with complete Freund's adjuvant. A suitable antiserum was obtained which showed no crossreactivity with eight naturally occurring peptides. N-Tyr-CRF was iodinated and used as tracer. With this assay, CRF-like immunoreactivity which coeluted with ovine CRF on Sephadex G50 was detected in rat hypothalami.

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R Moldow, R S Yalow
Dec 1, 1973·Urology·S R NewmarkA H Bennett
Mar 1, 1980·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R L Moldow, R S Yalow
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J SpiessW Vale
Dec 1, 1981·Brain Research Bulletin·R L MoldowC A Sandman
Jan 1, 1981·Peptides·C M TurkelsonM Shimizu

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Citations

Dec 1, 1982·Pharmacology, Biochemistry, and Behavior·A J KastinD H Coy
Sep 1, 1982·Peptides·A J Fischman, R L Moldow
Sep 1, 1987·Peptides·R L Moldow, A J Fischman
Dec 4, 1982·Lancet·A C Nieuwenhuijzen KrusemanG M Besser
Sep 1, 1988·The American Journal of Physiology·M GrafR L Moldow

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