PMID: 6979743Jan 1, 1981Paper

Radioimmunoassay of dermorphin-like peptides in mammalian and non-mammalian tissues

Peptides
L NegriV Erspamer

Abstract

A selective RIA for D-Ala2-Dermorphin (Der), a natural peptide extracted from amphibian skin, has been developed using an antibody raised in rabbits against Der which has been coupled to BSA through its phenolic hydroxyl groups of tyrosine residues with 2,4-Dichloro-6-methoxy-1,3,5-triazine. The cross-reactivity of this antibody with dermorphin analogs, C- and N-terminal fragments of dermorphin molecule, some opioid and gastrointestinal peptides was tested. Der-like immunoreactivity has been identified in tissue extracts of rats, frog and cephalopoda. Der-like peptides were purified by passing methanol extracts of the tissues through a Sephadex G25 column (16 x 100 cm) eluted with 0.1 M acetic acid at 4 degrees C. Der-like immunoreactivity from neural tissue of Dosidicus gigas, Eledone moscata, and rat brain showed a good agreement with an authentic sample of synthetic dermorphin.

References

Jul 1, 1981·British Journal of Pharmacology·M BroccardoP C Montecucchi

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Citations

Jan 1, 1982·Die Naturwissenschaften·R Schwyzer
Sep 1, 1993·Neuroscience and Behavioral Physiology·T N SollertinskayaM F Obukhova
Jul 16, 1990·Biochemical and Biophysical Research Communications·A MorP Nicolas
Mar 26, 1985·European Journal of Pharmacology·M BroccardoP Melchiorri
Sep 1, 1985·Metabolism: Clinical and Experimental·E C degli UbertiR Pansini
Jan 1, 1981·Peptides·V ErspamerT Renda
Jan 1, 1985·Peptides·R de Castiglione, A C Rossi
Jan 1, 1985·Peptides·E C Degli UbertiR Pansini
Oct 1, 1996·General Pharmacology·P Melchiorri, L Negri
Jan 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·V Erspamer
Jan 1, 1986·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·V ErspamerJ M Cei
Nov 9, 2000·Clinical and Experimental Pharmacology & Physiology·L LiuE Burcher
Mar 1, 1954·British Journal of Pharmacology and Chemotherapy·V ERSPAMER, G SALA
Jan 1, 1997·Annual Review of Biochemistry·G Kreil
Jan 1, 1988·Journal of Neural Transmission·A BerggrenU Sillén
Dec 1, 1992·Trends in Biochemical Sciences·A MorP Nicolas
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Jan 1, 1989·Neuropeptides·A MorP Pradelles
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Oct 22, 1986·Brain Research·C W Stevens, T L Yaksh
Nov 13, 1987·Biochemical and Biophysical Research Communications·M AmicheP Nicolas

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