PMID: 6105178Jul 1, 1980Paper

Radioimmunoassay of diazepam in samples of forensic interest

The Journal of Pharmacy and Pharmacology
M G Rutterford, R N Smith

Abstract

The radioimmunoassay of diazepam in biological fluids is described. The method is particularly applicable to samples encountered in forensic toxicology that may be haemolysed, decmposed and of small volume. The antiserum and tritiated diazepam that are used are commercially available. Polyethylene glycol (mol. wt 6000) is used to separate the bound and free fractons, and the free fracion is counted by extracting the tritiated diazepam into a toluene-based scintillant. Blood samples are extracted with ethyl acetate before analysis but urine samples may be assayed directly. The assay is convenient, quick and virtually specific for diazepam. The results correlate well with those obtained by gas chromatography.

References

Feb 1, 1978·The Journal of Pharmacy and Pharmacology·P J Howard
Jul 1, 1979·The Journal of Pharmacy and Pharmacology·P HuntJ P Raynaud
May 2, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·F OwenR C Bourne
Nov 1, 1971·The Journal of Clinical Endocrinology and Metabolism·B Desbuquois, G D Aurbach
Jul 1, 1980·The Journal of Pharmacy and Pharmacology·M G Rutterford, R N Smith

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Citations

Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shane R NeedhamWilliam D Korte
Jan 1, 1987·Drug Metabolism Reviews·J W Findlay
Jul 1, 1980·The Journal of Pharmacy and Pharmacology·M G Rutterford, R N Smith
Sep 1, 1983·The Journal of Pharmacy and Pharmacology·K Robinson, R N Smith

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