Radioimmunoassay of human pancreatic amylase with monoclonal antibody.

Annals of Clinical Biochemistry
C FujitaK Shima

Abstract

A monoclonal antibody (E-21) was obtained that specifically binds to human pancreatic amylase and shows negligible cross-reaction with human salivary amylase. Using this antibody a radioimmunoassay was developed for pancreatic amylase in human serum. The assay was shown to be sensitive (detectable up to 7 mg/L), reproducible, and specific for pancreatic amylase. In normal subjects, the mean concentration of serum pancreatic amylase determined by this method was 36.3 mg/L with a 95% confidence range of 16.5 to 79.2 mg/L. A good correlation was observed between the concentrations of immunoreactive pancreatic amylase (IR-PA) and enzymatic activities in 20 serum samples (r = 0.97). The concentration of serum IR-PA was below the detectable limit in pancreatectomised patients, and was greatly increased in patients with acute pancreatitis; the latter was accompanied by parallel changes in total enzymic activity. In patients with mumps, the serum IR-PA level was within the normal range whereas the total enzymic activity was elevated.

References

Oct 1, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y TakatsukaG Kosaki
Apr 1, 1978·Analytical Biochemistry·M Boehm-TruittA L Notkins
Aug 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·M T JalaliJ M Braganza
Jul 1, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·C ScullyJ A Beeley
Dec 1, 1983·Journal of Biochemistry·K Omichi, T Ikenaka

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Citations

Dec 30, 2014·Biochimica Et Biophysica Acta·Takanori MoriyamaNaoyuki Honma
Apr 22, 2017·The Cochrane Database of Systematic Reviews·Gianluca RompianesiKurinchi Selvan Gurusamy

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