PMID: 2509617Sep 1, 1989Paper

Radioimmunoassay of inhibin in various mammals

The Journal of Endocrinology
T HamadaM Igarashi

Abstract

A sensitive radioimmunoassay (RIA) for the determination of inhibin in peripheral plasma and tissue homogenates of different species has been developed using antisera to partially purified bovine follicular fluid (bFF) inhibin and 125I-labelled bFF 32 kDa inhibin. Antisera were produced by immunization of rabbits with partially purified bFF inhibin prepared by immunoaffinity chromatography. Increasing doses of a high titre antiserum could neutralize the suppressing effect of bFF, porcine follicular fluid and rat ovarian homogenate on FSH secretion from rat anterior pituitary cells in culture. Sensitivity of the assay was 3.1 ng International Research Standard of porcine inhibin per tube. Parallel inhibition curves were obtained for inhibin preparations from female and male animals of ten species, i.e. cattle, goats, sheep, cats, dogs, monkeys, pigs, horses, rats and man. Inhibin subunits and related proteins cross-reacted minimally with the antiserum used in the study. Plasma concentrations of inhibin in adult male and female rats were measured by the RIA before and at various times after gonadectomy. Inhibin levels in peripheral plasma before gonadectomy were significantly higher in adult female than in adult male rats. Inhibi...Continue Reading

Citations

Jan 24, 1998·Biochemical and Biophysical Research Communications·K TamuraH Kogo
Sep 27, 1993·Journal of Immunological Methods·W L WongJ A Lofgren
Jan 1, 1994·Domestic Animal Endocrinology·J F RoserE Hoye
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Jan 18, 2006·The Journal of Reproduction and Development·Longquan RenKazuyoshi Taya
Jul 29, 2006·The Journal of Reproduction and Development·Nobue KanematsuKazuyoshi Taya
Sep 2, 2006·The Journal of Reproduction and Development·Hiroyuki KanekoManabu Ozawa
Oct 30, 2007·The Journal of Reproduction and Development·Qiang WengKazuyoshi Taya
Nov 14, 2007·The Journal of Reproduction and Development·Manila SedqyarKazuyoshi Taya
Feb 2, 2008·The Journal of Reproduction and Development·Manila SedqyarKazuyoshi Taya
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Oct 16, 2008·The Journal of Reproduction and Development·Hiroyuki KanekoJunko Noguchi
Dec 22, 2012·The Journal of Reproduction and Development·ChunMei LiKazuyoshi Taya
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Nov 3, 2010·The Journal of Veterinary Medical Science·Saroch KaewmaneeKazuyoshi Taya
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Feb 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Hiroyuki KanekoNaomi Kashiwazaki
Sep 26, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Michiko NoguchiHiroyuki Kaneko

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