PMID: 6111086Dec 1, 1980Paper

Radioimmunoassay of MIF-1/Tyr-MIF-1-like material in rat pineal

Pharmacology, Biochemistry, and Behavior
A J KastinD H Coy

Abstract

The hypothalamic peptide MIF-1 (Pro-Leu-Gly-NH2) was coupled to thyroglobulin and injected into rabbits. The resulting antiserum reacted with the tetrapeptide Tyr-MIF-1 to a greater extent than with the tripeptide MIF-1, presumably because of a better conformation for antibody binding. By radioimmunoassay (RIA), immunoreactive MIF-1/Tyr-MIF-1-like material was found in the pineal gland of each of the 100 rats examined. The tendencies for slightly higher levels in pineals obtained from rats kept in constant darkness for two weeks, from rats in a normal light cycle decapitated at noon, or from rats which had been hypophysectomized were not statistically significant. Gel filtration of pineal extracts on a column of Sephadex G-10 revealed that by RIA one immunoreactive peak eluted near MIF-1 and oxytocin, and another peak near Tyr-MIF-1. The results suggest the presence in pineal tissue of an MIF-1-like material as well as a novel peptide containing Tyr-Pro-Leu-Gly-NH2 or a closely related structure for which oxytocin is unlikely to be the precursor.

Citations

Dec 1, 1981·Pharmacology, Biochemistry, and Behavior·R D OlsonD H Coy
Dec 1, 1981·Pharmacology, Biochemistry, and Behavior·A J KastinD H Coy
Dec 1, 1981·Pharmacology, Biochemistry, and Behavior·A J KastinD H Coy
Dec 1, 1982·Pharmacology, Biochemistry, and Behavior·J E ZadinaD H Coy
Dec 1, 1985·Pharmacology, Biochemistry, and Behavior·A J KastinA J Fischman
Jan 1, 1995·Brain Research Bulletin·L HacklerA J Kastin
Dec 1, 1984·Pharmacology, Biochemistry, and Behavior·A J KastinA J Fischman
Jun 1, 1992·International Journal of Peptide and Protein Research·J ErchegyiX D Qiu
Aug 1, 1990·Biochemical Pharmacology·W A Banks, A J Kastin
Mar 1, 1993·Neuropeptides·L HacklerJ E Zadina

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