PMID: 616043Apr 1, 1977Paper

Radioimmunoassay of secretin in plasma

Scandinavian Journal of Clinical and Laboratory Investigation
O B Schaffalitzky de Muckadell, J Fahrenkrug

Abstract

A sensitive and specific radioimmunoassay for secretin has been developed. 125I synthetic porcine secretin was used as label. Antibodies were raised against synthetic pocrine secretin. Pure natural porcine secretin was used as standard and free and bound hormone was separated by plasma-coated charcoal. Nonspecific interference with the assay system by plasma factors was abolished by extraction of plasma samples. The detection limit was 1.3 pmol/l. Within and between assay reproducibility (five triplicate determinations in each of eight assays) was 0.7 and 0.9 pmol/l respectivel (1 SD) at a concentration of 4.7 pmol/1. Fasting level in seventy normal persons ranged from less than 1.3 to 5.3 pmol/1. High concentrations were found in two patients with Zollinger-Ellison syndrome. In six normal persons intraduodenal acidification was immediately followed by an increase in secretin concentrations in peripheral venous blood to 7.7-23.6 pmol/1 (range), with a median of 15.5 pmol/1. In six anaesthetized pigs a fifty-fold increase in portal plasma secretin concentration after duodenal acidification was followed by a thirty-fold increased in the flow rate of pancreatic juice.

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Citations

Jan 1, 1992·Scandinavian Journal of Gastroenterology·L RasmussenS A Pedersen
Oct 1, 1990·European Journal of Clinical Investigation·J J Holst
Oct 1, 1988·Digestive Diseases and Sciences·J ChristiansenO B Schaffalitzky de Muckadell
Mar 1, 1985·Acta Physiologica Scandinavica·P G BurholR Jorde
Jan 1, 1983·Acta Physiologica Scandinavica·H A El MunshidR Håkanson
Jul 1, 1980·Digestive Diseases and Sciences·T M Chang, W Y Chey
Jan 1, 1981·Scandinavian Journal of Gastroenterology·H L WaldumP G Burhol
Aug 1, 1982·European Journal of Clinical Investigation·G R GreenbergS R Bloom
Mar 1, 1994·Scandinavian Journal of Gastroenterology·H GladO B Schaffalitzky de Muckadell
Jan 1, 1996·Scandinavian Journal of Gastroenterology. Supplement·J J HolstJ F Rehfeld
May 1, 1992·Scandinavian Journal of Gastroenterology·M A AinsworthO B Schaffalitzky de Muckadell
Jan 1, 1978·Scandinavian Journal of Gastroenterology·O B Schaffalitzky de MuckadellH Worning
Jul 1, 1993·Scandinavian Journal of Gastroenterology·O OlsenP Berlac
Mar 1, 1997·Scandinavian Journal of Gastroenterology·M WøjdemannJ F Rehfeld
Jan 1, 1983·European Journal of Applied Physiology and Occupational Physiology·O OktedalenO B Schaffalitzky de Muckadell
Jan 1, 1980·Scandinavian Journal of Gastroenterology·K G Wormsley
May 1, 1985·Digestive Diseases and Sciences·S BondesenO B Schaffalitzky de Muckadell
Jan 1, 1989·Scandinavian Journal of Gastroenterology·O OlsenP Cantor
Feb 19, 1998·The American Journal of Gastroenterology·M WøjdemannO Olsen
Oct 16, 1999·Scandinavian Journal of Gastroenterology·C RiberO Olsen
Jan 1, 1978·Scandinavian Journal of Gastroenterology·J G StageO B Muckadell
Sep 1, 1991·Annals of Nuclear Medicine·C H HsuP H Chen
May 1, 1989·Scandinavian Journal of Gastroenterology·J OlsenJ Christiansen
Jun 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·J MiskowiakO B Schaffalitzky de Muckadell
Mar 17, 2000·Scandinavian Journal of Gastroenterology·M WøjdemannO Olsen
Jul 15, 1999·The Journal of Clinical Endocrinology and Metabolism·M WøjdemannJ J Holst
Jan 1, 1981·Scandinavian Journal of Gastroenterology·O B Schaffalitzky de MuckadellH Worning
Oct 1, 1996·Scandinavian Journal of Gastroenterology·H GladO B Schaffalitzky de Muckadell
Oct 27, 2016·Journal of Neuroendocrinology·G Leng, N Sabatier
Sep 1, 1988·Scandinavian Journal of Gastroenterology·O OlsenH Worning
Oct 1, 1994·Scandinavian Journal of Gastroenterology·M A AinsworthO B Schaffalitzky de Muckadell
Jun 1, 1977·European Journal of Clinical Investigation·J Fahrenkrug, O B Schaffalitzky de Muckadell
May 1, 1979·The American Journal of Physiology·M S KimW Y Chey

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