PMID: 7515085May 16, 1994Paper

Radioimmunoassay versus flow cytometric assay to quantify LPS-binding protein (LBP) concentrations in human plasma

Journal of Immunological Methods
D HeumannM P Glauser

Abstract

LPS-binding protein (LBP) is an acute phase protein present in plasma, that has been shown to play a major role in sensitizing monocytes to LPS. We describe here two assays to quantify LBP in plasma. The first assay made use of monophosphoryl lipid A to capture LBP in human plasma, and LBP was detected by radiolabeled anti-LBP IgG. The second assay measured LBP by flow cytometry, using LBPs ability to present LPS to the CD14 receptor present on monocytes. Both assays had a similar level of sensitivity, that allowed the quantification of LBP in human plasma.

References

Dec 1, 1992·The Journal of Experimental Medicine·E A FreyS D Wright
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Sep 1, 1986·The Journal of Experimental Medicine·P S TobiasR J Ulevitch
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J PuginP S Tobias

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