PMID: 2509728Jan 1, 1989Paper

Radioimmunological determination of levonorgestrel and ethinylestradiol

Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao
Q G Li, B Nieuweboer

Abstract

A sensitive and specific radioimmunoassay for ethinylestradiol (EE2) and levonorgestrel (LN) has been developed. Antisera were raised in rabbits after injection of EE2-6-carboxymethyloxime-aminocaproic acid and LN-3-carboxy-methyloxime linked to bovine serum albumin. The sensitivity of immunoassay was 15 pg/ml for EE2 and 10 pg/ml for LN. The cross-reactions showed a high specificity of the assay, and the accuracy and precision of this method satisfied the requirements for the investigation of pharmacokinetics. With this assay, although the recovery of the assay was not very high (the coefficient of variation 14.02% for EE2 and 10.27% for LN) when used to analyse diethylether extracts of biological fluids, a microquantitative metabolite of norethisterone (NET) and norethisterone-3-oxime (NETO), EE2 analogue in incubation solution with rat hepatocytes, was satisfactorily detected. The result showed that in vitro the amount of NETO transferred to EE2 analogue was three times as much as that of NET. It is necessary to further establish the identity and activity of the analogue, but these data will be found advantageous to the research of steroid-oxime.

References

Jan 1, 1977·Hormone Research·B Nieuweboer, K Lübke
Jul 1, 1975·Journal of Steroid Biochemistry·S E Morris, E H Cameron
Jan 1, 1986·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·D Z LiB Z Xiao
Sep 1, 1974·The Journal of Endocrinology·R J Warren, K Fotherby
Jun 1, 1969·The Journal of Clinical Endocrinology and Metabolism·G E Abraham
May 1, 1964·Acta Endocrinologica·H OKADAG TOKUDA

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Citations

Jan 1, 1993·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·J P Shi, X R Wu
Jan 1, 1996·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·C WuD Huang
Oct 1, 1992·European Journal of Drug Metabolism and Pharmacokinetics·Q G Li, M Hümpel
Jun 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·Q G Li

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