Radioiodinated diacylglycerol analogue: a potential imaging agent for single-photon emission tomographic investigations of cerebral ischaemia

European Journal of Nuclear Medicine
Y OhmoriS Tazawa

Abstract

Phospholipid metabolism is closely related to membrane perturbation in cerebral ischaemia. We investigated in vivo topographical lipid metabolism using an iodine-123-labelled diacylglycerol analogue, (1-(15-(4-iodine-123-iodophenyl)-pentadecanoyl)-2-stearoyl-rac-gly cerol) (123I-labelled DAG), in a middle cerebral artery (MCA) occlusion model with the aim of positive imaging of ischaemic insult. Sprague-Dawley rats underwent coagulation of the MCA to induce permanent occlusion. MCA occlusion times prior to injection of 123I-labelled DAG ranged from 15 min to 14 days. Each rat was injected with 11-37 MBq of 123I-labelled DAG via a tail vein. After 30 min, in vivo autoradiographs were reconstructed. Scanning of the living rat brain in this MCA occlusion model was performed using a gamma camera with a pinhole collimator. Cerebral infarctions were recognized in the frontal cortex, the parietal cortex and the lateral portion of the caudate-putamen by 2,3,5-triphenyltetrazolium hydrochloride staining. In infarcted regions (region 1), 123I-labelled DAG incorporation showed a slight decrease up to 12 h; it then increased up to 6 days and decreased thereafter. In peri-infarcted regions (region 2), the incorporation showed almost no chan...Continue Reading

References

Feb 1, 1990·Trends in Biochemical Sciences·H KanohF Sakane
Jan 1, 1989·Annals of the New York Academy of Sciences·A A FarooquiL A Horrocks
Aug 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D Duverger, E T MacKenzie
Oct 1, 1987·Neurochemical Pathology·A A FarooquiL A Horrocks
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Feb 1, 1987·Journal of Neurochemistry·K AbeK Miyamoto
Jun 1, 1984·The Biochemical Journal·M J Berridge
May 1, 1982·The International Journal of Applied Radiation and Isotopes·D J HnatowichR L Childs
Oct 1, 1982·Journal of Neurochemistry·A D EdgarL A Horrocks
Jan 1, 1981·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A TamuraG M Teasdale
Jan 1, 1981·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A TamuraG M Teasdale
Jan 1, 1981·Progress in Lipid Research·L A HorrocksG Porcellati

❮ Previous
Next ❯

Citations

May 5, 2005·Veterinary Radiology & Ultrasound : the Official Journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·Kathelijne PeremansChristophe Van de Wiele

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.