Radioiodinated Peptidic Imaging Probes for in Vivo Detection of Membrane Type-1 Matrix Metalloproteinase in Cancers

Biological & Pharmaceutical Bulletin
Naoya KondoHideo Saji

Abstract

Membrane type-1 matrix metalloproteinase (MT1-MMP) plays pivotal roles in tumor progression and metastasis, and holds great promise as an early biomarker for malignant tumors. Therefore, the ability to evaluate MT1-MMP expression could be valuable for molecular biological and clinical studies. For this purpose, we aimed to develop short peptide-based nuclear probes because of their facile radiosynthesis, chemically uniform structures, and high specific activity, as compared to antibody-based probes, which could allow them to be more effective for in vivo MT1-MMP imaging. To the best of our knowledge, there have been no reports of radiolabeled peptide probes for the detection of MT1-MMP in cancer tissues. In this study, we designed and prepared four probes which consist of a MT1-MMP-specific binding peptide sequence (consisting of L or D amino acid isomers) and an additional cysteine (at the N or C-terminus) for conjugation with N-(m-[(123/125)I]iodophenyl) maleimide. We investigated probe affinity, probe stability in mice plasma, and probe biodistribution in tumor-bearing mice. Finally, in vivo micro single photon emission computed tomography (SPECT) imaging and ex vivo autoradiography were performed. Consequently, [(123)I]I-DC...Continue Reading

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Citations

Oct 3, 2017·Biological & Pharmaceutical Bulletin·Hideo Saji
Sep 19, 2018·Contrast Media & Molecular Imaging·Miguel Ángel MorcilloJorge Martínez-Torrecuadrada
Jan 7, 2020·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Naoya Kondo
Nov 28, 2019·European Journal of Nuclear Medicine and Molecular Imaging·Benjamin B KastenJason M Warram
Jun 7, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Roopali RoyMarsha A Moses

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