Abstract
Bendamustine is an important drug for the treatment of chronic lymphatic leukaemia (CLL), non-Hodgkin lymphoma (NHL). However, its delivery is challenging due to its instability. Current approach reports the development and characterization of bendamustine encapsulated PLGA nanoparticles for the effective targeting to leukemic cells. The prepared, bendamustine loaded PLGA nanoparticles (BLPNP) were developed and characterized for particle size, zeta potential and polydispersity index. The formed nanoparticles were further characterized with the help of electron microscopy for surface morphology. The formed nanoparticles were evaluated for cytotoxicity, cell uptake, ROS and cell apoptosis against THP-1 leukemic cells as a part of in vitro evaluation. In vivo organ bio-distribution and tumor regression studies were performed to track in vivo behaviour of BLPNP. The average particle size was 138.52 ± 3.25 nm, with 0.192 ± 0.036 PDI and - 25.4 ± 1.38 mV zeta potential. TEM images revealed the homogeneous particle size distribution with uniform shape. In vitro release exhibited a sustained drug-release behaviour up to 24 h. Cytotoxicity against THP-1 cells through MTT assay observed IC50 value of 27.8 ± 2.1 μM for BLPNP compared to ...Continue Reading
References
Apr 27, 2007·Biomaterials·Payal AgrawalN K Jain
Jun 19, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Hrushikesh B AgasheNarendra Kumar Jain
Oct 18, 2008·Journal of Pharmaceutical and Biomedical Analysis·Ivanka PenchevaKolio Troev
Oct 28, 2008·Bioconjugate Chemistry·Prateek SinghNarendra K Jain
May 20, 2009·ACS Nano·Atul PathakKailash C Gupta
Aug 28, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fabienne DanhierVéronique Préat
Oct 23, 2010·Advanced Drug Delivery Reviews·Sarbari Acharya, Sanjeeb K Sahoo
Aug 3, 2011·Biomaterials·Hai WangHao Chen
Nov 26, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Sachin NaikAmbikanandan Misra
Feb 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fabienne DanhierVéronique Préat
Oct 24, 2012·Advanced Drug Delivery Reviews·Ying ZhangKam W Leong
Dec 25, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sheva NaahidiP Chen
Feb 12, 2014·Molecular Pharmaceutics·Madhu GuptaSuresh Prasad Vyas
Jul 28, 2016·Colloids and Surfaces. B, Biointerfaces·Shindu C ThomasSushama Talegaonkar
Aug 17, 2016·International Journal of Biological Macromolecules·Iliyas KhanUmesh Gupta
Sep 22, 2016·Critical Reviews in Therapeutic Drug Carrier Systems·Iliyas KhanUmesh Gupta
Jan 16, 2017·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Yuvraj SinghManish K Chourasia
Feb 13, 2017·International Journal of Biological Macromolecules·Umesh GuptaVipin Kumar
Mar 30, 2017·International Journal of Nanomedicine·Jyoti BhandariSushama Talegaonkar
Jul 8, 2017·Pharmaceutical Research·Seema UpadhyayUmesh Gupta
Dec 2, 2017·Molecular Pharmaceutics·Avinash GothwalUmesh Gupta
Apr 1, 2018·Journal of Nanobiotechnology·Kalishwaralal KalimuthuMichael A Firer
Jun 23, 2018·Current Drug Delivery·Shindu C ThomasSushama Talegaonkar